Anticorps Monoclonal anti-GLUT1

• Phare
• Validé par KD/KO

GLUT1 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

2A5A2

N° de cat : CL594-66290

Synonymes

DYT17, DYT18, GLUT, GLUT 1, GLUT1, HepG2 glucose transporter, PED, SLC2A1

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Galerie de données de validation

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  IF Staining of human lung cancer using CL594-66290

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite®594 GLUT1 antibody (CL594-66290, Clone: 2A5A2 ) at dilution of 1:200.

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  IF Staining of human lung cancer using CL594-66290

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite®594 GLUT1 antibody (CL594-66290, Clone: 2A5A2 ) at dilution of 1:200.

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  FC experiment of Jurkat using CL594-66290

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human GLUT1 (CL594-66290, Clone:2A5A2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF: tissu de cancer du poumon humain,
• Résultats positifs en cytométrie: cellules Jurkat

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66290 cible GLUT1 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GLUT1 Protéine recombinante Ag17108
• Nom complet: solute carrier family 2 (facilitated glucose transporter), member 1
• Masse moléculaire calculée: 492 aa, 54 kDa
• Poids moléculaire observé: 45-55 kDa
• Numéro d’acquisition GenBank: BC121804
• Symbole du gène: SLC2A1
• Identification du gène (NCBI): 6513
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glucose transporter 1 (GLUT1), also known as solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1), is a uniporter protein responsible for the transport of glucose in many cell types and across the blood-brain barrier.

What is the molecular weight of GLUT1? Is GLUT1 post-translationally modified?

There are two forms of GLUT1 transporter that differ in their molecular weight. The 45-kDa form is found in glial cells, while the 55-kDa form is present in the endothelial cells regulating glucose transport over the blood-brain and blood-tissue barriers (PMID: 9630522). N-glycosylation of asparagine at position 42 is the only known post-translation modification of GLUT1 (PMID: 3839598).

What is the subcellular localization of GLUT1?

Glucose transporters, including GLUT1, are multiple-pass integral membrane proteins. GLUT1 is present at the plasma membrane but is also a subject of recycling between plasma membrane and endosomes.

What molecules can be transported by GLUT1?

The main substrate of GLUT1 transport is glucose, but it can also transport galactose, mannose, glucosamine, and reduced ascorbate. 

What is the tissue expression pattern of GLUT1?

GLUT1 is expressed by many cell types but the highest levels are observed in erythrocytes and in the central nervous system (astrocytes). GLUT1 is responsible for glucose transfer across the blood-brain and blood-tissue barriers, including placental transport.