Anticorps Polyclonal de lapin anti-GLTSCR2

• Phare
• Validé par KD/KO

GLTSCR2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 27353-1-AP

Synonymes

GLT, GLTSCR2, NOP53, p60, PICT 1, PICT1

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Galerie de données de validation

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  WB analysis of human placenta using 27353-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 27353-1-AP (GLTSCR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 27353-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27353-1-AP (GLTSCR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 27353-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27353-1-AP (GLTSCR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 27353-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27353-1-AP (GLTSCR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 27353-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27353-1-AP (GLTSCR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 27353-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using GLTSCR2 antibody (27353-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain,
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

27353-1-AP cible GLTSCR2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GLTSCR2 Protéine recombinante Ag26358
• Nom complet: glioma tumor suppressor candidate region gene 2
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC010095
• Symbole du gène: GLTSCR2
• Identification du gène (NCBI): 29997
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Biochem Biophys Res Commun; MTR4 adaptor PICT1 functions in two distinct steps during pre-rRNA processing; Authors - Sotaro Miyao ; KD Validated
human		bioRxiv; Nucleolar dynamics are determined by the ordered assembly of the ribosome; Authors - Jessica Sheu-Gruttadauria
human	WB	Dev Cell; Proteomic profiling of centrosomes across multiple mammalian cell and tissue types by an affinity capture method; Authors - Sarah Carden

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Elisa (Verified Customer) (03-15-2022)

U2OS cells were fixed in 4% PFA for 15' at Room temperature. Cells were washed once with PBS for. Membrane permeabilisation was then performed with 0.3% Triton in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at room temperature. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-555-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature). In this picture, cells are stained for Hoechst (DNA marker, in magenta) and GLTSCR2 (nucleolar protein in yellow).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: U2OS (Human Osteosarcoma) cells

GLTSCR2 Antibody Immunofluorescence validation (1:200 dilution) in U2OS (Human Osteosarcoma) cells (Cat no:27353-1-AP)