Anticorps Polyclonal de lapin anti-GLS

GLS Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 29519-1-AP

Synonymes

AAD20, DKFZp686O15119, FLJ10358, GAC, GLS, GLS1, glutaminase, K glutaminase, KIAA0838, L glutamine amidohydrolase

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Galerie de données de validation

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  WB analysis using 29519-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 29519-1-AP (GLS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of L02 using 29519-1-AP

  Immunofluorescent analysis of (4% PFA) fixed L02 cells using GLS antibody (29519-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HeLa, cellules MCF-7, cellules SH-SY5Y
• Résultats positifs en IF: cellules L02,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

29519-1-AP cible GLS dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GLS Protéine recombinante Ag31167
• Nom complet: glutaminase
• Masse moléculaire calculée: 73 kDa
• Poids moléculaire observé: 58 kDa, 65 kDa
• Numéro d’acquisition GenBank: NM_014905
• Symbole du gène: GLS
• Identification du gène (NCBI): 2744
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808).The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody can recognize KGA and GAC.

Publications

Species	Application	Title
mouse	WB	Nat Metab; Gut microbiome-derived ammonia modulates stress vulnerability in the host; Authors - Pei Wang
human	WB	Front Oncol; ITGB1-mediated molecular landscape and cuproptosis phenotype induced the worse prognosis in diffuse gastric cancer; Authors - Xingyu Zhu
human	WB	Front Pharmacol; A novel method for generating glutamatergic SH-SY5Y neuron-like cells utilizing B-27 supplement; Authors - Emily-Rose Martin
rat	WB	Gen Comp Endocrinol; Testosterone regulates thymic remodeling by altering metabolic reprogramming in male rats; Authors - Dong Li