Anticorps Polyclonal de lapin anti-GFP tag

GFP tag Polyclonal Antibody for IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Protéine recombinante, Aequorea Victoria

Applications

IF

Conjugaison

CoraLite®594 Fluorescent Dye

N° de cat : CL594-50430

Synonymes

---

Galerie de données de validation

• 

  IF Staining of Transfected HEK-293 using CL594-50430

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite®594 GFP tag antibody (CL594-50430) at dilution of 1:200.

---

Applications testées

• Résultats positifs en IF: cellules HEK-293 transfectées,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-50430 cible GFP tag dans les applications de IF et montre une réactivité avec des échantillons Protéine recombinante, Aequorea Victoria

• Réactivité: Protéine recombinante, Aequorea Victoria
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GFP tag Protéine recombinante Ag2128
• Nom complet: GFP tag
• Masse moléculaire calculée: 26 kDa
• Numéro d’acquisition GenBank: U73901
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). This antibody is a rabbit polyclonal antibody raised against full-length eGFP and reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP and eGFP.