Anticorps Monoclonal anti-GFAP

GFAP Monoclonal Antibody for IF

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

IF

Conjugaison

CoraLite®594 Fluorescent Dye

Publications

1

CloneNo.

4B2E10

N° de cat : CL594-60190

Synonymes

FLJ45472, GFAP

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Galerie de données de validation

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  IF Staining of mouse brain using CL594-60190

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL594-60190 (GFAP antibody) at dilution of 1:100.

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  IF Staining of mouse brain using CL594-60190

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL594-60190 (GFAP antibody) at dilution of 1:100.

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Applications testées

• Résultats positifs en IF: tissu cérébral de souris,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

This antibody is not recommended for immunocytofluorescent assays. It is not suitable for frozen sections.

Applications publiées

• IF: See 1 publications below

Informations sur le produit

CL594-60190 cible GFAP dans les applications de IF et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GFAP Protéine recombinante Ag10452
• Nom complet: glial fibrillary acidic protein
• Masse moléculaire calculée: 432 aa, 50 kDa
• Numéro d’acquisition GenBank: BC013596
• Symbole du gène: GFAP
• Identification du gène (NCBI): 2670
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GFAP Function GFAP (Glial fibrillary acidic protein) is a type III intermediate filament (IF) protein specific to the central nervous system (CNS). GFAP is one of the main components of the intermediate filament network in astrocytes and has been proposed as playing a role in cell migration, cell motility, maintaining mechanical strength, and in mitosis. Tissue specificity GFAP is expressed in central nervous system cells, predominantly in astrocytes. GFAP is commonly used as an astrocyte marker. However, GFAP is also present in peripheral glia and in non-CNS cells, including fibroblasts, chondrocytes, lymphocytes, and liver stellate cells (PMID: 21219963). Involvement in disease Mutations in GFAP lead to Alexander disease (OMIM: 203450), an autosomal dominant CNS disorder. The mutations present in affected individuals are thought to be gain-of-function. Upregulation of GFAP is a hallmark of reactive astrocytes, in which GFAP is present in hypertrophic cellular processes. Reactive astrogliosis is present in many neurological disorders, such as stroke, various neurodegenerative diseases (including Alzheimer's and Parkinson's disease), and neurotrauma. Isoforms Astrocytes express 10 different isoforms of GFAP that differ in the rod and tail domains (PMID: 25726916), which means that they differ in molecular size. Isoform expression varies during the development and across different subtypes of astrocytes. Not all isoforms are upregulated in reactive astrocytes. Post-translational modifications Intermediate filament proteins are regulated by phosphorylation. Six phosphorylation sites have been identified in GFAP protein, at least some of which are reported to control filament assembly (PMID: 21219963). Cellular localization GFAP localizes to intermediate filaments and stains well in astrocyte cellular processes. The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Publications

Species	Application	Title
mouse	IF	Glia; Mechanosensitive channel Piezo1 is an essential regulator in cell cycle progression of optic nerve head astrocytes; Authors - Yue Wan