Anticorps Monoclonal anti-GFAP

GFAP Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

Publications

4

CloneNo.

4B2E10

N° de cat : CL488-60190

Synonymes

GFAP

---

Galerie de données de validation

• 

  IF Staining of mouse brain using CL488-60190

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-60190 (GFAP antibody) at dilution of 1:100.

• 

  IF Staining of mouse brain using CL488-60190

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-60190 (GFAP antibody) at dilution of 1:100.

• 

  FC experiment of Jurkat using CL488-60190

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GFAP (CL488-60190, Clone:4B2E10) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en IF: tissu cérébral de souris,
• Résultats positifs en cytométrie: cellules Jurkat

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:200
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

This antibody is not recommended for immunocytofluorescent assays. It is not suitable for frozen sections.

Applications publiées

• IF: See 4 publications below

Informations sur le produit

CL488-60190 cible GFAP dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GFAP Protéine recombinante Ag10452
• Nom complet: glial fibrillary acidic protein
• Masse moléculaire calculée: 432 aa, 50 kDa
• Numéro d’acquisition GenBank: BC013596
• Symbole du gène: GFAP
• Identification du gène (NCBI): 2670
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GFAP (Glial fibrillary acidic protein) is a type III intermediate filament (IF) protein specific to the central nervous system (CNS). GFAP is one of the main components of the intermediate filament network in astrocytes and has been proposed as playing a role in cell migration, cell motility, maintaining mechanical strength, and in mitosis. GFAP is expressed in central nervous system cells, predominantly in astrocytes. GFAP is commonly used as an astrocyte marker. However, GFAP is also present in peripheral glia and in non-CNS cells, including fibroblasts, chondrocytes, lymphocytes, and liver stellate cells (PMID: 21219963). Astrocytes express 10 different isoforms of GFAP that differ in the rod and tail domains (PMID: 25726916), which means that they differ in molecular size. Isoform expression varies during the development and across different subtypes of astrocytes. Not all isoforms are upregulated in reactive astrocytes. Intermediate filament proteins are regulated by phosphorylation. Six phosphorylation sites have been identified in GFAP protein, at least some of which are reported to control filament assembly (PMID: 21219963). GFAP localizes to intermediate filaments and stains well in astrocyte cellular processes. This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Publications

Species	Application	Title
mouse	IF	J Neurosci Res; Neuronal deficiency of hypoxia-inducible factor 2α increases hypoxic-ischemic brain injury in neonatal mice.; Authors - Dawei Sun
mouse	IF	Front Pharmacol; Extracellular Vesicle-Mediated Delivery of Ultrasmall Superparamagnetic Iron Oxide Nanoparticles to Mice Brain.; Authors - Naseer A Kutchy
rat	IF	Brain Res; Effects of cannabinoid (CBD) on blood brain barrier permeability after brain injury in rats.; Authors - Hongyan Jiang
mouse	IF	Neural Regen Res; Transient neurogenesis in ischemic cortex from Sox2+ astrocytes; Authors - Jia-Lei Yang