Anticorps Monoclonal anti-GDI1

GDI1 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

4E1G1

N° de cat : 66434-1-Ig

Synonymes

GDI 1, GDI1, GDIL, GDP dissociation inhibitor 1, MRX41, MRX48, Oligophrenin 2, OPHN2, Protein XAP 4, Rab GDI alpha, RABGD1A, RABGDIA, XAP 4, XAP4

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Galerie de données de validation

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  WB analysis of U-251 using 66434-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 66434-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 66434-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 66434-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66434-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66434-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66434-1-Ig (GDI1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66434-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66434-1-Ig (GDI1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 66434-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66434-1-Ig (GDI1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules U-251, cellules HEK-293, cellules Neuro-2a, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochimie (IHC): IHC : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 2 publications below

Informations sur le produit

66434-1-Ig cible GDI1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GDI1 Protéine recombinante Ag17511
• Nom complet: GDP dissociation inhibitor 1
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC000317
• Symbole du gène: GDI1
• Identification du gène (NCBI): 2664
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family. GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI1 is expressed primarily in neural and sensory tissues and also in secretory cells, displaying a diffuse, cytoplasmic distribution in cells. It runs as a 55 kDa protein in SDS-PAGE. (PMID: 7929030,PMID: 19570034)

Publications

Species	Application	Title
human	IHC	Bioengineered; Overexpression of GDP dissociation inhibitor 1 gene associates with the invasiveness and poor outcomes of colorectal cancer; Authors - Xiao Xie
human	IHC	Signal Transduct Target Ther; Erianin, a novel dibenzyl compound in Dendrobium extract, inhibits lung cancer cell growth and migration via calcium/calmodulin-dependent ferroptosis.; Authors - Peng Chen