Anticorps Polyclonal de lapin anti-GDI1

GDI1 Polyclonal Antibody for WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB,ELISA

Conjugaison

Non conjugué

N° de cat : 55214-1-AP

Synonymes

GDI 1, GDI1, GDIL, GDP dissociation inhibitor 1, MRX41, MRX48, Oligophrenin 2, OPHN2, Protein XAP 4, Rab GDI alpha, RABGD1A, RABGDIA, XAP 4, XAP4

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Galerie de données de validation

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  WB analysis of SH-SY5Y using 55214-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55214-1-AP (GDI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 55214-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55214-1-AP (GDI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 55214-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55214-1-AP (GDI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55214-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55214-1-AP (GDI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55214-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55214-1-AP (GDI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 55214-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55214-1-AP (GDI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, tissu cérébral de souris, tissu cérébral humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

55214-1-AP cible GDI1 dans les applications de WB,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: GDP dissociation inhibitor 1
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 51-55 kDa
• Numéro d’acquisition GenBank: NM_001493
• Symbole du gène: GDI1
• Identification du gène (NCBI): 2664
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family. GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI1 is expressed primarily in neural and sensory tissues and also in secretory cells, displaying a diffuse, cytoplasmic distribution in cells. It runs as a 55kda protein in SDS-PAGE. (PMID: 7929030,PMID: 19570034). Defects in GDI1 are the cause of mental retardation X-linked type 41 (MRX41). Defects in GDI1 are the cause of mental retardation X-linked type 48 (MRX48). The antibody is specific to GDI1. And it has no cross reaction to GDI2.