Anticorps Polyclonal de lapin anti-GDF15

• Phare
• Validé par KD/KO

GDF15 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

23

N° de cat : 27455-1-AP

Synonymes

GDF 15, GDF15, MIC 1, MIC1, NAG 1, NRG 1, NSAID activated gene 1 protein, NSAID regulated gene 1 protein, PDF, PLAB, Placental TGF beta, PTGFB

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Galerie de données de validation

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  WB analysis using 27455-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27455-1-AP (GDF15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 27455-1-AP

  WB result of GDF15 antibody (27455-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GDF15 transfected HepG2 cells.

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  WB analysis using 27455-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 27455-1-AP (GDF15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 27455-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 27455-1-AP (GDF15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 27455-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27455-1-AP (GDF15 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 27455-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27455-1-AP (GDF15 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse placenta using 27455-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 27455-1-AP (GDF15 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse placenta using 27455-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 27455-1-AP (GDF15 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HT-1080, cellules HepG2, cellules LNCaP, tissu placentaire humain
• Résultats positifs en IHC: tissu placentaire humain, tissu placentaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 12 publications below
• WB: See 22 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

27455-1-AP cible GDF15 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GDF15 Protéine recombinante Ag26760
• Nom complet: growth differentiation factor 15
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC008962
• Symbole du gène: GDF15
• Identification du gène (NCBI): 9518
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Growth differentiation factor 15 (GDF15), also known as macrophage inhibitory cytokine-1 (MIC-1), is a protein of the transforming growth factor beta (TGFb) superfamily that regulates inflammatory and apoptotic pathways in injured tissues and during disease processes. GDF15 is a stress-induced cytokine and associated with hypoxia, inflammation and oxidative stress, it is also released from endothelial cells after stimulation with pro-inflammatory cytokines. GDF15 has been suggested as a target and biomarker for cardiovascular disease as it plays a cardioprotective role in the adult heart. GDF15 is highly expressed in the placenta. GDF15 is known to be involved in human embryo development and necessary for the maintenance of pregnancy. GDF15 is also produced by adipocytes in response to oxidative stress, a well-known factor associated with preeclampsia.

Publications

Species	Application	Title
human,mouse	WB	Gastroenterology; Hepatic Stellate Cells in Hepatocellular Carcinoma Promote Tumor Growth Via Growth Differentiation Factor 15 Production.; Authors - Yuta Myojin ; KO Validated
human	WB	Nat Commun; Dual functions of SPOP and ERG dictate androgen therapy responses in prostate cancer.; Authors - Tiziano Bernasocchi
human,mouse	WB,IHC	Cancer Lett; SULF2 enhances GDF15-SMAD axis to facilitate the initiation and progression of pancreatic cancer.; Authors - Ruizhe He
rat	WB,IF	Oxid Med Cell Longev; Cyclic Polypeptide D7 Protects Bone Marrow Mesenchymal Cells and Promotes Chondrogenesis during Osteonecrosis of the Femoral Head via Growth Differentiation Factor 15-Mediated Redox Signaling.; Authors - Jiazheng Chen
human	WB	Cancers (Basel); GDF15 Is an Eribulin Response Biomarker also Required for Survival of DTP Breast Cancer Cells.; Authors - Chiara Bellio ; KD Validated
human	WB	Antioxidants (Basel); Grape Seed Proanthocyanidins Induce Apoptosis and Cell Cycle Arrest of HepG2 Cells Accompanied by Induction of the MAPK Pathway and NAG-1.; Authors - Lihua Wang