Anticorps Monoclonal anti-GAPDH

GAPDH Monoclonal Antibody for FC (Intra), WB

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, levure, plante, poisson-zèbre, rat, souris

Applications

WB, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

Publications

3

CloneNo.

1E6D9

N° de cat : CL594-60004

Synonymes

G3PD, GAPD, GAPDH, OK/SW cl.12

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Galerie de données de validation

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  WB analysis using CL594-60004

  Various lysates were subjected to SDS PAGE followed by western blot with CL594-60004 (GAPDH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using CL594-60004

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human GAPDH (CL594-60004, Clone:1E6D9) (red), or 0.2 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules Jurkat
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

CL594-60004 cible GAPDH dans les applications de WB, FC (Intra) et montre une réactivité avec des échantillons Humain, levure, plante, poisson-zèbre, rat, souris

• Réactivité: Humain, levure, plante, poisson-zèbre, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GAPDH Protéine recombinante Ag0766
• Nom complet: glyceraldehyde-3-phosphate dehydrogenase
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC004109
• Symbole du gène: GAPDH
• Identification du gène (NCBI): 2597
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Publications

Species	Application	Title
mouse	WB	Brain Sci; Itaconate Attenuates Neuroinflammation and Exerts Dopamine Neuroprotection in Parkinson's Disease through Inhibiting NLRP3 Inflammasome; Authors - Guoqing Sun
human	WB	Cell Chem Biol; Identification of KLHDC2 as an efficient proximity-induced degrader of K-RAS, STK33, β-catenin, and FoxP3; Authors - Sascha Röth
mouse	WB	Int J Mol Med; Flavin containing monooxygenase 2 regulates renal tubular cell fibrosis and paracrine secretion via SMURF2 in AKI‑CKD transformation; Authors - Longfei Wang

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C