Anticorps Monoclonal anti-GAPDH

GAPDH Monoclonal Antibody for FC (Intra), WB

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, levure, plante, poisson-zèbre, rat, souris

Applications

WB, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

Publications

7

CloneNo.

1E6D9

N° de cat : CL488-60004

Synonymes

G3PD, GAPD, GAPDH, OK/SW cl.12

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Galerie de données de validation

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  WB analysis using CL488-60004

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-60004 (GAPDH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using CL488-60004

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-60004 (GAPDH antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using CL488-60004

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GAPDH (CL488-60004, Clone:1E6D9) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules Jurkat
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below

Informations sur le produit

CL488-60004 cible GAPDH dans les applications de WB, FC (Intra) et montre une réactivité avec des échantillons Humain, levure, plante, poisson-zèbre, rat, souris

• Réactivité: Humain, levure, plante, poisson-zèbre, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GAPDH Protéine recombinante Ag0766
• Nom complet: glyceraldehyde-3-phosphate dehydrogenase
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC004109
• Symbole du gène: GAPDH
• Identification du gène (NCBI): 2597
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Publications

Species	Application	Title
human	WB	Aging (Albany NY); Coagulation factor 2 thrombin receptor promotes malignancy in glioma under SOX2 regulation.; Authors - Guojun Gao
human	WB	Cell Commun Signal; Integral membrane protein 2A inhibits cell growth in human breast cancer via enhancing autophagy induction.; Authors - Cefan Zhou
human	WB	Sci Rep; Comparative analysis of pathophysiological parameters between emphysematous smokers and emphysematous patients with COPD.; Authors - Shuang Bai
human	WB	Exp Cell Res; CLEC12B suppresses lung cancer progression by inducing SHP-1 expression and inactivating the PI3K/AKT signaling pathway.; Authors - Decai Chi
rat	WB	Virus Res; Interaction of porcine reproductive and respiratory syndrome virus major envelope proteins GP5 and M with the cellular protein Snapin.; Authors - Julie A Hicks
mouse	WB	Reprod Fertil Dev; Effects of 2,3',4,4',5-pentachlorobiphenyl exposure during pregnancy on DNA methylation in the testis of offspring in the mouse.; Authors - Jian-Mei Zhang

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C