Anticorps Monoclonal anti-G3BP2

G3BP2 Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

2E5G3

N° de cat : 68580-1-Ig

Synonymes

G3BP 2, G3BP2, KIAA0660

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Galerie de données de validation

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  WB analysis of SCaBER using 68580-1-Ig

  SCaBER cells were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68580-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 68580-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 68580-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HCT 116 using 68580-1-Ig

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68580-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 68580-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP2 antibody (68580-1-Ig, Clone: 2E5G3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HCT 116, cellules HepG2, cellules HT-29, cellules K-562, cellules MOLT-4, tissu cérébral de rat
• Résultats positifs en IF: sodium arsenite treated HeLa cells,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68580-1-Ig cible G3BP2 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: G3BP2 Protéine recombinante Ag9222
• Nom complet: GTPase activating protein (SH3 domain) binding protein 2
• Masse moléculaire calculée: 482aa,54 kDa; 449aa,51 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC011731
• Symbole du gène: G3BP2
• Identification du gène (NCBI): 9908
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation. They function to protect RNAs from harmful conditions. G3BP2 is mainly distributed in the cytoplasm and participates in the formation of stress granules, cell differentiation, proliferation, and signal transduction. Accumulating evidence has demonstrated that aberrant expression of G3BP2 contributes to cancer initiation and progression, such as high expression of G3BP2 increasing cell stemness, metastasis and chemoresistance in breast cancer.