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Anticorps Monoclonal anti-G3BP1

G3BP1 Monoclonal Antibody for IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

IF

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E4A2

N° de cat : CL488-66486

Synonymes

G3BP, G3BP 1, G3BP1, HDH VIII

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated G3BP1 Monoclonal ant (CL488-66486)

    IF Staining of HeLa using CL488-66486

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using CoraLite® Plus 488 G3BP1 antibody (CL488-66486, Clone: 1E4A2 ) at dilution of 1:200, CL594-Phalloidin (red).

Applications testées

Résultats positifs en IFsodium arsenite treated HeLa cells,

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-66486 cible G3BP1 dans les applications de IF et montre une réactivité avec des échantillons Humain, porc, rat, souris

Réactivité Humain, porc, rat, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène G3BP1 Protéine recombinante Ag3728
Nom complet GTPase activating protein (SH3 domain) binding protein 1
Masse moléculaire calculée 466 aa, 52 kDa
Poids moléculaire observé 68 kDa
Numéro d’acquisition GenBankBC006997
Symbole du gène G3BP1
Identification du gène (NCBI) 10146
Conjugaison CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.

Protocole

Product Specific Protocols
IF protocol for CL Plus 488 G3BP1 antibody CL488-66486Download protocol
Standard Protocols
Click here to view our Standard Protocols

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Tatyana (Verified Customer) (01-21-2023)

Excellent bright signal only after 30 min incubation (1:1000) at RT. PFA-fixed cells were used. Labels sodium arsenite induced stress granules well.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human / U2OS cells
CL Plus 488 G3BP1 Antibody Immunofluorescence validation (1:1000 dilution) in Human / U2OS cells (Cat no:CL488-66486)