Anticorps Monoclonal anti-Frizzled 9

Frizzled 9 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

1G9G4

Avis (1)

N° de cat : 67023-1-Ig

Synonymes

CD349, Frizzled 9, Fz 9, FZD3, FZD9, FzE6, hFz9

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Galerie de données de validation

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  WB analysis of A549 using 67023-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 67023-1-Ig (Frizzled 9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NCI-H1299 using 67023-1-Ig

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 67023-1-Ig (Frizzled 9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 67023-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 67023-1-Ig (Frizzled 9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67023-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67023-1-Ig (Frizzled 9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 67023-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 67023-1-Ig (Frizzled 9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 67023-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67023-1-Ig (Frizzled 9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 67023-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67023-1-Ig (Frizzled 9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 67023-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using Frizzled 9 antibody (67023-1-Ig, Clone: 1G9G4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HUVEC, cellules NCI-H1299, tissu cérébral humain fœtal
• Résultats positifs en IHC: tissu de gliome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67023-1-Ig cible Frizzled 9 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Frizzled 9 Protéine recombinante Ag4971
• Nom complet: frizzled homolog 9 (Drosophila)
• Masse moléculaire calculée: 591 aa, 64 kDa
• Poids moléculaire observé: 64-70 kDa
• Numéro d’acquisition GenBank: BC026333
• Symbole du gène: FZD9
• Identification du gène (NCBI): 8326
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; Cross-linking of the endolysosomal system reveals potential flotillin structures and cargo; Authors - Jasjot Singh

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Susanne (Verified Customer) (04-17-2023)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Fibroblasts

Frizzled 9 Antibody Immunofluorescence validation (1:500 dilution) in Fibroblasts (Cat no:67023-1-Ig)