Anticorps Monoclonal anti-Foxp3

Foxp3 Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

Publications

1

CloneNo.

3G3

N° de cat : CL488-65089

Synonymes

forkhead box P3, Foxp3, JM2, scurfin, sf

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Galerie de données de validation

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  FC experiment of mouse splenocytes using CL488-65089

  1X10^6 mouse splenocytes were surface stained with CoraLite® Plus 647-conjugated Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5) and then fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were then stained with CoraLite® Plus 488-conjugated mouse IgG1 isotype control or 5 ul CoraLite® Plus 488-conjugated Anti-Mouse Foxp3 (CL488-65089, Clone: 3G3).

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-65089 cible Foxp3 dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: forkhead box P3
• Numéro d’acquisition GenBank: BC132333
• Symbole du gène: Foxp3
• Identification du gène (NCBI): 20371
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Foxp3 (forkhead box P3), also named as IPEX, JM2 and scurfin, is a member of the forkhead transcription factor family and is essential for normal immune homeostasis (PMID: 11138001; 20429413). Foxp3 is specifically expressed in CD4+ CD25+ regulatory T cells and is crucial for their development and suppressive activity (PMID: 12612578; 12612581). In scurfy mouse, disruption of Foxp3 results in the fatal lymphoproliferative disorder attributed to the lack of an immune-suppressive component in the immune system (PMID: 25683611; 11138001). Similar to mice, genetic defects in human Foxp3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome (PMID: 11137993).

Publications

Species	Application	Title
mouse	FC (Intra)	Adv Sci (Weinh); Cancer-Associated Fibroblast-Induced Remodeling of Tumor Microenvironment in Recurrent Bladder Cancer; Authors - Ting Liang

Produits associés

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011