Anticorps Monoclonal anti-DYKDDDDK Tag

DYKDDDDK Tag Monoclonal Antibody for WB

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Protéine recombinante et plus (1)

Applications

WB

Conjugaison

HRP

Publications

13

CloneNo.

8H6A10

N° de cat : HRP-66008

Synonymes

DDDDK tag, DYKDDDDK, DYKDDDDK Tag, HRP conjugated DDDDK tag, HRP-66008, HRP-DDDDK tag

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Galerie de données de validation

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  WB analysis of Recombinant protein using HRP-66008

  Recombinant protein were subjected to SDS PAGE followed by western blot with HRP-66008 (DYKDDDDK Tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: Protéine recombinante

Dilution recommandée

• Western Blot (WB): WB : 1:10000-1:100000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below

Informations sur le produit

HRP-66008 cible DYKDDDDK Tag dans les applications de WB et montre une réactivité avec des échantillons Protéine recombinante

• Réactivité: Protéine recombinante
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: DYKDDDDK Tag
• Conjugaison: HRP
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DYKDDDDK tag, also known as DDK tag, is a hydrophilic tag for recombinant protein technology with the sequence DYKDYKDDDDK. The structure of DYKDDDDK tag has been optimized for compatibility with the proteins it is attached to and unlikely to denature or inactivated them. For its high hydrophilic nature, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag more likely to be accessible to antibodies. This product is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, no matter it is/they are located at N-terminal, C-terminal or at internal regions. The binding of this product with DYKDDDDK tagged fusion protein does not depend on calcium ion. The clone of this monoclonal antibody hybridoma is 1E7B4, which is different from any other vendors. This antibody is HRP conjugated DYKDDDDK Tag antibody. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Publications

Species	Application	Title
	WB	Nat Commun; FAM222A encodes a protein which accumulates in plaques in Alzheimer's disease.; Authors - Tingxiang Yan
	WB	Liver Int; The DEAD-box helicase DDX3x ameliorates non-alcoholic fatty liver disease via mTORC1 signalling pathway.; Authors - Peihao Liu
		Biomed Pharmacother; PMID: 32036219; Authors - Yu Chen
		Biochem Biophys Res Commun; Vaccination with a DNA vaccine encoding CD317-targeting HBs antigen elicits enhanced immunity in mice.; Authors - Guizhong Zhang
	WB	J Bacteriol; Shigella flexneri Diguanylate Cyclases Regulate Virulence.; Authors - Ruchi Ojha
	WB	Biochem Biophys Res Commun; Harnessing coronavirus spike proteins' binding affinity to ACE2 receptor through a novel baculovirus surface display system.; Authors - Lin Wang