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Anticorps Monoclonal anti-FXR1

FXR1 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1E12E8

N° de cat : CL647-67813

Synonymes

FXR1, hFXR1p

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 647-conjugated FXR1 Monoclonal anti (CL647-67813)

    IF Staining of mouse brain using CL647-67813

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 FXR1 antibody (CL647-67813, Clone: 1E12E8 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 647-conjugated FXR1 Monoclonal anti (CL647-67813)

    FC experiment of HeLa using CL647-67813

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human FXR1 (CL647-67813, Clone:1E12E8) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en IFtissu cérébral de souris,
Résultats positifs en cytométriecellules HeLa

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-67813 cible FXR1 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène FXR1 Protéine recombinante Ag27800
Nom complet fragile X mental retardation, autosomal homolog 1
Masse moléculaire calculée 621 aa, 70 kDa
Poids moléculaire observé70-80 kDa
Numéro d’acquisition GenBankBC028983
Symbole du gène FXR1
Identification du gène (NCBI) 8087
Conjugaison CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tumour necrosis factor-alpha (TNF-alpha) is a key mediator of inflammation in host defence against infection and in autoimmune disease. Its production is controlled post-transcriptionally by multiple RNA-binding proteins that interact with the TNF-alpha AU-rich element and regulate its expression; Fragile X mental retardation-related protein 1 (FXR1) is one of these. FXR1 (fragile-X-mental retardation-related protein 1) are RNA-binding proteins that have been demonstrated to impact miRNA-mediated, post-transcriptional gene regulation, and required for embryonic and postnatal development of muscle tissue. It can regulate intracellular transport and local translation of certain mRNAs. The FXR1 exists some isoforms with the MV 69 kDa and 60 kDa.

Protocole

Product Specific Protocols
IF protocol for CL Plus 647 FXR1 antibody CL647-67813Download protocol
FC protocol for CL Plus 647 FXR1 antibody CL647-67813Download protocol
Standard Protocols
Click here to view our Standard Protocols