Anticorps Polyclonal de lapin anti-FUS/TLS

FUS/TLS Polyclonal Antibody for FC (Intra), IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite®488 Fluorescent Dye

N° de cat : CL488-11570

Synonymes

75 kDa DNA pairing protein, FUS, FUS1, Oncogene FUS, Oncogene TLS

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Galerie de données de validation

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  IF Staining of HepG2 using CL488-11570

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®488 FUS/TLS antibody (CL488-11570) at dilution of 1:200.

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  IF Staining of HepG2 using CL488-11570

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®488 FUS/TLS antibody (CL488-11570) at dilution of 1:200.

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  IF Staining of HeLa using CL488-11570

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®488 FUS/TLS antibody (CL488-11570) at dilution of 1:200, CoraLite®594 TDP-43 (C-terminal) antibody (CL594-12892, red).

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  FC experiment of MCF-7 using CL488-11570

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug CoraLite®488 Anti-Human FUS/TLS (CL488-11570) (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IF: cellules HepG2, cellules HeLa
• Résultats positifs en cytométrie: cellules MCF-7,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-11570 cible FUS/TLS dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FUS/TLS Protéine recombinante Ag2150
• Nom complet: fusion (involved in t(12;16) in malignant liposarcoma)
• Masse moléculaire calculée: 75 kDa
• Numéro d’acquisition GenBank: BC026062
• Symbole du gène: FUS
• Identification du gène (NCBI): 2521
• Conjugaison: CoraLite®488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 491 nm / 516 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration[PMID: 22640227]. FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) and is implicated in certain forms of amyotrophic lateral sclerosis (ALS) and frontotemporal dementias (FTDs) such as frontotemporal lobar dementia with ubiquitin inclusions (FTLD-U)[PMID: 22640227]. This antibody is a rabbit polyclonal antibody raised against an internal region of human FUS. This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Produits associés

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011