Anticorps Monoclonal anti-FUCA1

FUCA1 Monoclonal Antibody for IF

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, souris

Applications

IF

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

1G5C5

N° de cat : CL594-67251

Synonymes

Alpha L fucosidase 1, Alpha L fucosidase I, FUCA, FUCA1, fucosidase, alpha L 1, tissue, Tissue alpha L fucosidase

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Galerie de données de validation

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  IF Staining of human liver cancer using CL594-67251

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 FUCA1 antibody (CL594-67251, Clone: 1G5C5 ) at dilution of 1:200. DAPI (blue).

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  IF Staining of human liver cancer using CL594-67251

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 FUCA1 antibody (CL594-67251, Clone: 1G5C5 ) at dilution of 1:200. DAPI (blue).

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Applications testées

• Résultats positifs en IF: tissu de cancer du foie humain,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-67251 cible FUCA1 dans les applications de IF et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FUCA1 Protéine recombinante Ag9638
• Nom complet: fucosidase, alpha-L- 1, tissue
• Masse moléculaire calculée: 461 aa, 53 kDa
• Poids moléculaire observé: 50-56 kDa
• Numéro d’acquisition GenBank: BC017338
• Symbole du gène: FUCA1
• Identification du gène (NCBI): 2517
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FUCA1(a-l-Fucosidase) is a lysosomal glycosidase which is ubiquitous in eukaryotic cells. This enzyme is usually found as a soluble component of the lysosome and functions as an acid hydrolase in the degradation of numerous and diverse fucoglycoconjugates.The mammalian a-l-fucosidases are relatively large, multisubunit glycoproteins which exist in multiple molecular isoforms.This enzyme may have a role in sperm-egg interactions in the reproductive tract of the female rat. There is a precursor 54 kDa form of rat sperm fucosidase which is processed to a mature 52 kDa form of the enzyme(PMID:8836125). This protein from rat epididymis is a tetramer of 210-220 kDa, made up by two pairs of subunits of 47 and 60 kDa, and the one from human seminal plasma exists in different oligomeric forms, that is, as a dimer, as a tetramer, and as a hexamer made up of a single subunit of 56-57 kDa(PMID:16736526).