Anticorps Monoclonal anti-FOXN1

FOXN1 Monoclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

1D8B12

N° de cat : 66337-1-Ig

Synonymes

FKHL20, forkhead box N1, Forkhead box protein N1, FOXN1, RONU, WHN

---

Galerie de données de validation

• 

  WB analysis of Raji using 66337-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66337-1-Ig (FOXN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66337-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66337-1-Ig (FOXN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of A549 using 66337-1-Ig

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human FOXN1 (66337-1-Ig, Clone:1D8B12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

---

Applications testées

• Résultats positifs en WB: cellules Raji, cellules Jurkat
• Résultats positifs en cytométrie: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66337-1-Ig cible FOXN1 dans les applications de WB, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FOXN1 Protéine recombinante Ag18207
• Nom complet: forkhead box N1
• Masse moléculaire calculée: 648 aa, 69 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC146539
• Symbole du gène: FOXN1
• Identification du gène (NCBI): 8456
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FOXN1, also named as Forkhead box protein N1, is a 648 amino acid protein, which contains 1 fork-head DNA-binding domain and localizes in the nucleus. FOXN1 as transcriptional regulator regulates the development, differentiation, and function of thymic epithelial cells (TECs) both in the prenatal and postnatal thymus. FOXN1 acts as a master regulator of the TECs lineage development and is required from the onset of differentiation in progenitor TECs in the developing fetus to the final differentiation steps through which TECs mature to acquire their full functionality.