Anticorps Monoclonal anti-NeutraKine FLT3 Ligand

NeutraKine FLT3 Ligand Monoclonal Antibody for Neutralization, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

Neutralization, ELISA

Conjugaison

Non conjugué

CloneNo.

3E1A3

N° de cat : 69032-1-Ig

Synonymes

FL, Flt3 ligand, Flt3L, FLT3LG, SL cytokine

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Galerie de données de validation

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  Neutralization experiment of NeutraKine FLT3 Ligand using 69032-1-Ig

  Recombinant human FLT3 Ligand (Cat.NO. HZ-1151) stimulates proliferation of OCI-AML5 cells in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human FLT3 (1 ng/mL) is neutralized by mouse anti-human FLT3 Ligand monoclonal antibody 69032-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 0.01-0.05 μg/mL.The control mouse anti-human FLT3 Ligand monoclonal antibody 69532-1-Ig could recognize human FLT3 Ligand, but could not neutralize human FLT3 Ligand at serial does at the range of 0.12207-2000ng/mL(green curve, refer to top X-right Y).

• 

  Neutralization experiment of NeutraKine FLT3 Ligand using 69032-1-Ig

  Recombinant human FLT3 Ligand (Cat.NO. HZ-1151) stimulates proliferation of OCI-AML5 cells in a dose dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human FLT3 Ligand (1ng/mL HZ-1151) is neutralized by mouse anti-human FLT3 Ligand monoclonal antibody 69032-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 0.01-0.05 μg/mL.

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  ELISA experiment of Recombinant protein using 69032-1-Ig

  Indirect ELISA was carried out by coating recombinant Human FLT3 Ligand (Cat.NO. HZ-1151) at 70 ng/well followed by blocking and adding serial diluted FLT3 Ligand antibody 69032-1-Ig and 69532-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

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• Neutralization: OCI-AML5

Informations sur le produit

69032-1-Ig cible NeutraKine FLT3 Ligand dans les applications de Neutralization, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: human NeutraKine FLT3 Ligand protein HZ-1151
• Nom complet: fms-related tyrosine kinase 3 ligand
• Symbole du gène: FLT3LG
• Identification du gène (NCBI): 2323
• Conjugaison: Non conjugué
• Forme: Lyophilized Powder
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS stérile.
• Endotoxin: <0.1 EU/μg
• Reconstitution: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Informations générales

The FMS-related tyrosine kinase 3 (FLT3) ligand (FLT3LG), a growth factor, is mainly derived from lymphocytes in the tumor microenvironment. It binds to FLT3 on DCs to enhance their differentiation and expansion. Therefore, it may assist DCs in tumor antigen presentation and antitumor immune response. In addition, mature DCs express FLT3 and proliferate massively under the trigger of FLT3LG.

This antibody is used to neutralize the bioactivity of FLT3 Ligand.