Anticorps Monoclonal anti-Flightless I

Flightless I Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

2F9C8

N° de cat : 67039-1-Ig

Synonymes

FLI, Fli1, Flightless I, FLII, FLIL, Protein flightless 1 homolog

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Galerie de données de validation

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  WB analysis using 67039-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67039-1-Ig (FLII antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67039-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67039-1-Ig (FLII antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 67039-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67039-1-Ig (FLII antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67039-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67039-1-Ig (FLII antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules HT-1080, cellules Jurkat, cellules LNCaP, cellules MCF-7, cellules NCCIT, cellules NCI-H1299, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

67039-1-Ig cible Flightless I dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Flightless I Protéine recombinante Ag26865
• Nom complet: flightless I homolog (Drosophila)
• Masse moléculaire calculée: 1269 aa, 145 kDa
• Poids moléculaire observé: 145-150 kDa
• Numéro d’acquisition GenBank: BC025300
• Symbole du gène: FLII
• Identification du gène (NCBI): 2314
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Flightless I (FliI) is the most evolutionarily conserved member of the gelsolin superfamily of proteins which are key regulators of actin filament assembly and turnover. FliI comprises an N-terminal leucine-rich repeat (LRR) domain which is not present in other gelsolin family members, and the LRR domain may enable interactions between FliI and other molecules involved in signal transduction, thereby spatially integrating signaling and actin remodeling functions. This protein was originally found in Drosophila and participates in the embryonic development, while mammalian FliI protein was involved in the regulation of wound repair,skin barrier development. Studies recently demonstrated that FliI protein associated with colorectal cancer, hepatocellular and prostate cancer (PMID:30091651; 28498392).

Publications

Species	Application	Title
	WB	Genes Dev; Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein; Authors - Megan L Norris
human	WB	Am J Cancer Res; SH3BGRL2 exerts a dual function in breast cancer growth and metastasis and is regulated by TGF-β1.; Authors - Dou-Dou Li