Anticorps Monoclonal anti-FKBP52

FKBP52 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF, FC

Conjugaison

Non conjugué

CloneNo.

3F5F9

N° de cat : 66040-1-Ig

Synonymes

51 kDa FK506 binding protein, 52 kDa FK506 binding protein, 52 kDa FKBP, 59 kDa immunophilin, FK506 binding protein 4, FK506 binding protein 4, 59kDa, FKBP 4, FKBP 52, FKBP4, FKBP51, FKBP52, FKBP59, HBI, HSP binding immunophilin, Hsp56, Immunophilin FKBP52, p52, p59, PPIase, PPIase FKBP4, Rotamase

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Galerie de données de validation

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  WB analysis of HeLa using 66040-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66040-1-Ig (FKBP52 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66040-1-Ig

  MCF7 cells were subjected to SDS PAGE followed by western blot with 66040-1-Ig (FKBP52 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 66040-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66040-1-Ig (FKBP52 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66040-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66040-1-Ig (FKBP52 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66040-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66040-1-Ig (FKBP52 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 66040-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66040-1-Ig (FKBP52 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

• 

  IHC staining of human cervical cancer using 66040-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66040-1-Ig (FKBP52 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

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  IHC staining of human colon cancer using 66040-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66040-1-Ig (FKBP52 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

• 

  IHC staining of human colon cancer using 66040-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66040-1-Ig (FKBP52 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

• 

  IF Staining of HeLa using 66040-1-Ig

  Immunofluorescent analysis of HeLa cells using 66040-1-Ig (FKBP52 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

• 

  FC experiment of HeLa using 66040-1-Ig

  1X10^6 HeLa cells were stained with 0.2ug FKBP52 antibody (66040-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

• 

  FC experiment of Jurkat using 66040-1-Ig

  1X10^6 Jurkat cells were stained with 0.20ug FKBP52 antibody (66040-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules K-562, cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa, cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66040-1-Ig cible FKBP52 dans les applications de WB, IHC, IF, FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FKBP52 Protéine recombinante Ag18704
• Nom complet: FK506 binding protein 4, 59kDa
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC007924
• Symbole du gène: FKBP4
• Identification du gène (NCBI): 2288
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FKBP52 (FK506 binding protein 4) is a member of the immunophilin protein family that comprises intracellular protein effectors of immunosuppressive drugs, and it is known as a steroid receptor-associated protein. As a novel regulator of microtubule dynamics, FKBP52 is associated with the motor protein dynein and with the cytoskeleton during mitosis. FKBP52 is highly expressed in CNS regions susceptible to Alzheimer's, and plays a role in modulating toxicity of Abeta peptides. The protein can associate with the Tau function, and may help to decipher and modulate the events involved in Tau-induced neurodegeneration. In addition, FKBP52 is likely to play a role in growth and development of the male genitalia.