Anticorps Monoclonal anti-FGL2

FGL2 Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

2C9C5

N° de cat : 67152-1-Ig

Synonymes

FGL2, FGL-2, fibrinogen like 2, Fibrinogen like protein 2, Fibroleukin, pT49, T49

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Galerie de données de validation

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  WB analysis of THP-1 using 67152-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67152-1-Ig (FGL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67152-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67152-1-Ig (FGL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse spleen using 67152-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using FGL2 antibody (67152-1-Ig, Clone: 2C9C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse spleen using 67152-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using FGL2 antibody (67152-1-Ig, Clone: 2C9C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules THP-1, cellules Jurkat
• Résultats positifs en IF: tissu splénique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67152-1-Ig cible FGL2 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FGL2 Protéine recombinante Ag28619
• Nom complet: fibrinogen-like 2
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC017813
• Symbole du gène: FGL2
• Identification du gène (NCBI): 10875
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FGL2 (fibrinogen like protein 2) is a 70 kDa glycoprotein that belongs to the fibrinogen-related superfamily of proteins which are involved in coagulation, cell adhesion, and transendothelial migration. FGL2 is an important immune regulator of both innate and adaptive response. It is present on the surface of macrophages and endothelial cells, and can be constitutively secreted by CD4+ CD8+ T cell. FGL2 forms a tetrameric complex which is stabilized by interchain disulfide bonds and it exerts immunosuppressive effects on both T cell proliferation and dendritic cell maturation. The protein may play a role in physiologic functions at mucosal sites.

Publications

Species	Application	Title
human	WB	J Clin Invest; Targeting fibrinogen-like protein 1 enhances immunotherapy in hepatocellular carcinoma; Authors - Mingen Lin