Anticorps Monoclonal anti-FGL1

FGL1 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

2B11C6

N° de cat : 67391-1-Ig

Synonymes

FGL1, fibrinogen like 1, Fibrinogen like protein 1, Hepassocin, HFREP 1, HFREP1, HP 041, LFIRE 1, LFIRE1

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Galerie de données de validation

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  WB analysis of pig liver using 67391-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67391-1-Ig (FGL1 antibody) at dilution of 1:2700 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 67391-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67391-1-Ig (FGL1 antibody) at dilution of 1:2700 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 67391-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67391-1-Ig (FGL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 67391-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67391-1-Ig (FGL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 67391-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 67391-1-Ig (FGL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 67391-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 67391-1-Ig (FGL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67391-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67391-1-Ig (FGL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67391-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67391-1-Ig (FGL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67391-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FGL1 antibody (67391-1-Ig, Clone: 2B11C6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using 67391-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human FGL1 (67391-1-Ig, Clone:2B11C6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Applications testées

• Résultats positifs en WB: tissu hépatique de porc, tissu placentaire humain
• Résultats positifs en IHC: tissu hépatique humain, tissu de cancer du foie humain, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5400
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67391-1-Ig cible FGL1 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FGL1 Protéine recombinante Ag8581
• Nom complet: fibrinogen-like 1
• Masse moléculaire calculée: 312 aa, 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC007047
• Symbole du gène: FGL1
• Identification du gène (NCBI): 2267
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fibrinogen-like 1 (FGL1), also termed hepatocyte-derived fibrinogen-related protein 1 (HFREP1) or hepassocin, is a predominantly liver expressed protein that has been implicated as both a hepatoprotectant and a hepatocyte mitogen. FGL1 expression is decreased in hepatocellular carcinoma (HCC), and it may play a role in the development of hepatocellular carcinomas.

Publications

Species	Application	Title
mouse	WB	Mol Ther Oncolytics; Dual-targeting vaccine of FGL1/CAIX exhibits potent anti-tumor activity by activating DC-mediated multi-functional CD8 T cell immunity; Authors - Dafei Chai