Anticorps Monoclonal anti-FFAR3

FFAR3 Monoclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

1D10B7

N° de cat : 66811-1-Ig

Synonymes

FFA3R, FFAR3, free fatty acid receptor 3, G protein coupled receptor 41, GPR41

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Galerie de données de validation

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  WB analysis using 66811-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66811-1-Ig (FFAR3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66811-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66811-1-Ig (FFAR3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human adipose using 66811-1-Ig

  human adipose tissue were subjected to SDS PAGE followed by western blot with 66811-1-Ig (FFAR3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66811-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66811-1-Ig (FFAR3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 66811-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 66811-1-Ig (FFAR3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HT-29, tissu adipeux humain
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

66811-1-Ig cible FFAR3 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FFAR3 Protéine recombinante Ag28025
• Nom complet: free fatty acid receptor 3
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 40-48 kDa
• Numéro d’acquisition GenBank: BC035657
• Symbole du gène: FFAR3
• Identification du gène (NCBI): 2865
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FFAR3 (Free fatty acid receptors 3) also known as GPR41, with another GPR43 protein, both are Gai-coupled receptor activated by short-chain fatty acids (SCFAs) such as acetate, propionate, and butyrate (PMID:26870043). GPR41 protein is translated from the bicistronic mRNA encoding GPR40 and GPR41, where an internal ribosome entry site (IRES) is utilized for the GPR41 coding sequence downstream of GPR40 (PMID:22493486). GPR41 is expressed in adipose tissue, gut, and the peripheral nervous system, and it is involved in SCFA-dependent energy regulation(PMID: 24904531).

Publications

Species	Application	Title
human	IF	Front Immunol; Natural fish oil improves the differentiation and maturation of oligodendrocyte precursor cells to oligodendrocytes in vitro after interaction with the blood-brain barrier; Authors - Paweł Piatek
human	WB,IF	Front Microbiol; Gut microbiome and serum short-chain fatty acids are associated with responses to chemo- or targeted therapies in Chinese patients with lung cancer; Authors - Huan-Huan Chen
human	WB	Gut Microbes; Butyrate reduces adherent-invasive E. coli-evoked disruption of epithelial mitochondrial morphology and barrier function: involvement of free fatty acid receptor 3; Authors - Samira A Hamed