Anticorps Polyclonal de lapin anti-FBP17

FBP17 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 27056-1-AP

Synonymes

FBP17, FNBP1, formin binding protein 1, Formin binding protein 17, hFBP17, KIAA0554

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Galerie de données de validation

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  WB analysis of mouse brain using 27056-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27056-1-AP (FBP17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 27056-1-AP

  IP Result of anti-FBP17 (IP:27056-1-AP, 4ug; Detection:27056-1-AP 1:1000) with mouse brain tissue lysate 3000ug.

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  IHC staining of human lung cancer using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27056-1-AP (FBP17 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung cancer using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27056-1-AP (FBP17 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27056-1-AP (FBP17 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27056-1-AP (FBP17 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27056-1-AP (FBP17 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27056-1-AP (FBP17 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27056-1-AP (FBP17 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27056-1-AP (FBP17 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris,
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu cardiaque de souris, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:150-1:600
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

27056-1-AP cible FBP17 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FBP17 Protéine recombinante Ag25784
• Nom complet: formin binding protein 1
• Poids moléculaire observé: 80-85 kDa
• Numéro d’acquisition GenBank: BC101755
• Symbole du gène: FNBP1
• Identification du gène (NCBI): 23048
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FBP17 (formin-binding protein 17) is a new dynamin-associating molecule consisting of several functional domains, including an N-terminal EFC domain and an SH3 domain at the C-terminus. FBP17 is involved in dynamin-mediated endocytosis and has been reported to be required for invadopodia formation and tumor cell invasion.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

María (Verified Customer) (02-08-2022)

Works fine for WB (1:1000), for IF some background, even after several washes with detergent

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hela/RPE-1/MEF/NaTi