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Anticorps Polyclonal de lapin anti-FBF1

FBF1 Polyclonal Antibody for IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IF

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

N° de cat : CL488-11531

Synonymes

Alb, albatross, Fas (TNFRSF6) binding factor 1, FBF 1, FBF1, FBF1 Ens, FLJ00103, FLJ13996, HCG1989313, isoform CRA_b, KIAA1863

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite® Plus 488-conjugated FBF1 Polyclonal anti (CL488-11531)

    IF Staining of A549 using CL488-11531

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 FBF1 antibody (CL488-11531) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using CoraLite® Plus 488-conjugated FBF1 Polyclonal anti (CL488-11531)

    IF Staining of HUVEC using CL488-11531

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CoraLite® Plus 488 FBF1 antibody (CL488-11531) at dilution of 1:200.

Applications testées

Résultats positifs en IFcellules A549, cellules HUVEC

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-11531 cible FBF1 dans les applications de IF et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène FBF1 Protéine recombinante Ag2106
Nom complet Fas (TNFRSF6) binding factor 1
Masse moléculaire calculée1133 aa, 125 kDa
Poids moléculaire observé 130 kDa
Numéro d’acquisition GenBankBC023549
Symbole du gène FBF1
Identification du gène (NCBI) 85302
Conjugaison CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FBF1, also named as ALB and KIAA1863, is keratin-binding protein required for epithelial cell polarization. It is involved in apical junction complex (AJC) assembly via its interaction with PARD3. FBF1 is required for ciliogenesis. FBF1 has six isoforms with MW 125-127 kDa, 99 kDa, 69 kDa and 55 kDa. This antibody is induced by N-terminal (20-347aa) of FBF1. It recognizes all the six isoforms.

Protocole

Product Specific Protocols
IF protocol for CL Plus 488 FBF1 antibody CL488-11531Download protocol
Standard Protocols
Click here to view our Standard Protocols

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Karine (Verified Customer) (02-26-2024)

FBF1-Coralite488 (in green) does label one extremity of the primary cilium labeled with ARL13B (in red) in RPE1 cells whose nuclei appear in purple and cell contour in grey with Brightfield. It is quite specific when used at 1/1000 overnight at 4°C. There is a bit more aspecific signal than with the uncoupled antibdy but it remains quite reasonable.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: RPE1 cells
CL Plus 488 FBF1 Antibody Immunofluorescence validation (1/1000 dilution) in RPE1 cells (Cat no:CL488-11531)