Anticorps Polyclonal de lapin anti-FAM13A

• Phare
• Validé par KD/KO

FAM13A Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 55401-1-AP

Synonymes

FAM13A, FAM13A1, KIAA0914, Protein FAM13A

---

Galerie de données de validation

• 

  WB analysis of mouse kidney using 55401-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55401-1-AP (FAM13A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 55401-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 55401-1-AP (FAM13A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 55401-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 55401-1-AP (FAM13A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 55401-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 55401-1-AP (FAM13A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 55401-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55401-1-AP (FAM13A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 55401-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55401-1-AP (FAM13A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 55401-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55401-1-AP (FAM13A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 55401-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55401-1-AP (FAM13A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsil using 55401-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 55401-1-AP (FAM13A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsil using 55401-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 55401-1-AP (FAM13A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsil using 55401-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 55401-1-AP (FAM13A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsil using 55401-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 55401-1-AP (FAM13A antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 55401-1-AP

  Immunofluorescent analysis of HeLa cells using 55401-1-AP (FAM13A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: tissu rénal de souris, tissu ovarien de souris, tissu pancréatique de souris, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de muscle squelettique humain, tissu amygdalien humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

55401-1-AP cible FAM13A dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: family with sequence similarity 13, member A
• Masse moléculaire calculée: 117 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: NM_014883
• Symbole du gène: FAM13A
• Identification du gène (NCBI): 10144
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FAM13A, also named as FAM13A1 and KIAA0914, belongs to the FAM13 family. It is associated with chronic obstructive pulmonary disease.(PMID: 20173748) This antibody is specific to FAM13A. It can recognize all the isoforms of FAM13A.

Publications

Species	Application	Title
mouse	WB	J Immunol; Annexin A2 Regulates Autophagy in Pseudomonas aeruginosa Infection through the Akt1-mTOR-ULK1/2 Signaling Pathway.; Authors - Rongpeng Li
	WB, IHC	Front Physiol; A Protective Role of FAM13A in Human Airway Epithelial Cells Upon Exposure to Cigarette Smoke Extract.; Authors - Qing Chen
human	WB	Mol Med Rep; miR‑30a‑5p induces the adipogenic differentiation of bone marrow mesenchymal stem cells by targeting FAM13A/Wnt/β‑catenin signaling in aplastic anemia.; Authors - Enbo Wang ; KD Validated
human	IHC	Am J Physiol Lung Cell Mol Physiol; FAM13A regulates cellular senescence marker p21 and mitochondrial reactive oxygen species production in airway epithelial cells; Authors - Qing Chen
human	IF	Am J Respir Cell Mol Biol; AKT Phosphorylates FAM13A and Promotes Its Degradation via CUL4A/DDB1/DCAF1 E3 Complex; Authors - Lu Gong