Anticorps Polyclonal de lapin anti-FABP7

FABP7 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 51010-1-AP

Synonymes

B FABP, BLBP, Brain lipid binding protein, FABP7, FABPB, Fatty acid binding protein 7, MRG

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Galerie de données de validation

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  WB analysis using 51010-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51010-1-AP (FABP7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 51010-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 51010-1-AP (FABP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 51010-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 51010-1-AP (FABP7 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 51010-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 51010-1-AP (FABP7 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 51010-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 51010-1-AP (FABP7 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 51010-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 51010-1-AP (FABP7 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human malignant melanoma using 51010-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 51010-1-AP (FABP7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human malignant melanoma using 51010-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 51010-1-AP (FABP7 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 51010-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 51010-1-AP (FABP7 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 51010-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 51010-1-AP (FABP7 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of rat brain using 51010-1-AP

  Immunofluorescent analysis of (-20°C Acetone) fixed rat brain tissue using FABP7 antibody (51010-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain fœtal, tissu adipeux de souris, tissu cérébral humain
• Résultats positifs en IHC: tissu de mélanome malin humain, tissu cérébral humain, tissu de cancer du sein humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de rat,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

51010-1-AP cible FABP7 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: fatty acid binding protein 7, brain
• Masse moléculaire calculée: 132aa,15 kDa; 166aa,19 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC012299
• Symbole du gène: FABP7
• Identification du gène (NCBI): 2173
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FABP7 (brain fatty acid-binding protein; BLBP) is a member of the FABP family of lipid chaperones involved in the uptake and intracellular trafficking of fatty acids. FABP7 is expressed in radial glial cells during brain development. FABP7 is up-regulated in brain tumor tissue; its expression has been associated with decreased survival times and tumor progression in patients with astrocytoma, melanoma, basal-type breast cancer, and renal cell carcinoma. This antibody is useful to label radial gial cells.

Publications

Species	Application	Title
mouse	WB	ACS Nano; Nanointegrative In Situ Reprogramming of Tumor-Intrinsic Lipid Droplet Biogenesis for Low-Dose Radiation-Activated Ferroptosis Immunotherapy; Authors - Qiqi Zhang
mouse	WB	Biomaterials; MMP-12 siRNA improves the homeostasis of the small intestine and metabolic dysfunction in high-fat diet feeding-induced obese mice.; Authors - Mingming Song
human	IF	Nat Commun; Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors.; Authors - Reuven Edri
mouse	IHC	Cancer Res; Bcl-xL enforces a slow-cycling state necessary for survival in the nutrient-deprived microenvironment of pancreatic cancer.; Authors - Yogev Sela
rat	WB	Int J Biol Macromol; Soybean soluble polysaccharide prevents obesity in high-fat diet-induced rats via lipid metabolism regulation; Authors - Haiyan He
mouse	IHC	Front Immunol; RGC-32 Regulates Generation of Reactive Astrocytes in Experimental Autoimmune Encephalomyelitis.; Authors - Alexandru Tatomir