Anticorps Polyclonal de lapin anti-FABP5

• Phare
• Validé par KD/KO

FABP5 Polyclonal Antibody for FC (Intra), IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite®555 Fluorescent Dye

N° de cat : CL555-12348

Synonymes

E FABP, EFABP, FABP5, Fatty acid binding protein 5, PA FABP, PAFABP

---

Galerie de données de validation

• 

  IF Staining of HeLa using CL555-12348

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®555 FABP5 antibody (CL555-12348) at dilution of 1:200.

• 

  IF Staining of HeLa using CL555-12348

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®555 FABP5 antibody (CL555-12348) at dilution of 1:200.

• 

  FC experiment of HeLa using CL555-12348

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human FABP5 (CL555-12348) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of HeLa using CL555-12348

  1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®555 Anti-Human FABP5 (CL555-12348) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-12348 cible FABP5 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FABP5 Protéine recombinante Ag3005
• Nom complet: fatty acid binding protein 5 (psoriasis-associated)
• Masse moléculaire calculée: 135 aa, 15 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC019385
• Symbole du gène: FABP5
• Identification du gène (NCBI): 2171
• Conjugaison: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FABP5, also named as PA-FABP and E-FABP, belongs to the calycin superfamily and Fatty-acid binding protein (FABP) family. It is high specificity for fatty acids. FABP5 is highest affinity for C18 chain length. It may be involved in keratinocyte differentiation. FABP5 is a fatty acid-binding protein and is expressed in epidermis and endothelial cells of the microvasculature of different organs. FABP5 has also been identified as a tumor-associated antigen, which is highly expressed in various cancers. FABP5 was detected in the sera of HNSCC patients with early stage cancer. Antibodies specific for FABP5 were significantly increased in a substantial amount in patients, suggesting that FABP5 may be a potential diagnostic biomarker for HNSCC. FABP5 may serve as a biomarker for HNSCC.(PMID:19602232)

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C