Anticorps Polyclonal de lapin anti-F4/80

F4/80 Polyclonal Antibody for FC, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris et plus (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

149

Avis (1)

N° de cat : 28463-1-AP

Synonymes

Adgre1, DD7A5 7, EGF TM7, Emr1, F4 80, F4/80, Gpf480, Ly71, TM7LN3

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Galerie de données de validation

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  IHC staining of mouse spleen using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat spleen using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 28463-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using F4/80 antibody (28463-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse spleen using 28463-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using F4/80 antibody (28463-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of RAW 264.7 using 28463-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using F4/80 antibody (28463-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse liver using 28463-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using 28463-1-AP (F4/80 antibody) at dilution of 1:500 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of RAW 264.7 using 28463-1-AP

  1X10^6 RAW 264.7 cells were intracellularly stained with 0.4 ug Anti-Mouse F4/80 (28463-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IHC: tissu splénique de souris, tissu cardiaque de souris, tissu cérébral de souris, tissu d'intestin grêle de souris, tissu hépatique de rat, tissu hépatique de souris, tissu pulmonaire de souris, tissu splénique de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu splénique de souris, cellules RAW 264.7, tissu hépatique de souris
• Résultats positifs en cytométrie: cellules RAW 264.7,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:4000-1:12000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 12 publications below
• IHC: See 79 publications below
• IF: See 56 publications below
• FC: See 8 publications below

Informations sur le produit

28463-1-AP cible F4/80 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: F4/80 Protéine recombinante Ag28252
• Nom complet: EGF-like module containing, mucin-like, hormone receptor-like sequence 1
• Masse moléculaire calculée: 102 kDa
• Numéro d’acquisition GenBank: NM_010130
• Symbole du gène: Emr1
• Identification du gène (NCBI): 13733
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mouse F4/80, also named as EMR1 and Gpf480, is a 160kd cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.

Publications

Species	Application	Title
mouse	FC	Biomaterials; Light-controlled oxygen production and collection for sustainable photodynamic therapy in tumor hypoxia.; Authors - Haoran Wang
pig	IF	Biomaterials; A myogenic niche with a proper mechanical stress environment improves abdominal wall muscle repair by modulating immunity and preventing fibrosis.; Authors - Shengyu Wang
mouse	IHC	Adv Healthc Mater; Silk Acid as an Implantable Biomaterial for Tissue Regeneration; Authors - Ziyang Sun
mouse	IHC	Aging Dis; P-Glycoprotein Aggravates Blood Brain Barrier Dysfunction in Experimental Ischemic Stroke by Inhibiting Endothelial Autophagy; Authors - Liangliang Huang
mouse	IHC	Autophagy; Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis.; Authors - Jingru Bai
rat	IHC	Acta Biomater; An electrospun scaffold functionalized with a ROS-scavenging hydrogel stimulates ocular wound healing; Authors - Xin Shi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kannan (Verified Customer) (06-13-2023)

AR using EDTApH9. overnight incubation of primary antibody at 4c.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mouse uterus

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C