Anticorps Polyclonal de lapin anti-Factor XII

Factor XII Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC,ELISA

Conjugaison

Non conjugué

N° de cat : 27154-1-AP

Synonymes

Coagulation factor XII, F12, Factor XII, HAE3, HAEX, HAF, Hageman factor

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Galerie de données de validation

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  WB analysis of human plasma using 27154-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 27154-1-AP (F12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 27154-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 27154-1-AP (F12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 27154-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27154-1-AP (F12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 27154-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27154-1-AP (F12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 27154-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27154-1-AP (F12 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 27154-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27154-1-AP (F12 antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu plasmatique humain, cellules HepG2, cellules Jurkat, cellules K-562
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

27154-1-AP cible Factor XII dans les applications de WB, IHC,ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Factor XII Protéine recombinante Ag25912
• Nom complet: coagulation factor XII (Hageman factor)
• Masse moléculaire calculée: 615 aa, 68 kDa
• Poids moléculaire observé: 80 kDa and 52 kDa
• Numéro d’acquisition GenBank: BC012390
• Symbole du gène: F12
• Identification du gène (NCBI): 2161
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Factor XII (F XII, Hageman factor) is a 80kD, single chain glycoprotein that circulates in blood as an inactive zymogen. F XII plays an important role in blood coagulation, fibrinolysis, and kinin generation. Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of a 52 kDa NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a 28 kDa COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2.