Anticorps Monoclonal anti-EXOSC2

EXOSC2 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

CloneNo.

1G8B1

Avis (2)

N° de cat : 66099-1-Ig

Synonymes

EXOSC2, exosome component 2, hRrp4p, p7, RRP4, Rrp4p

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Galerie de données de validation

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  WB analysis using 66099-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66099-1-Ig (EXOSC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis of HeLa using 66099-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66099-1-Ig (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66099-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66099-1-Ig (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66099-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66099-1-Ig (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 66099-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer slide using 66099-1-Ig (EXOSC2 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 66099-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66099-1-Ig (EXOSC2 Antibody) at dilution of 1:50.

• 

  IHC staining of human skin cancer using 66099-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer slide using 66099-1-Ig (EXOSC2 Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 66099-1-Ig

  Immunofluorescent analysis of () fixed HepG2 cells using 66099-1-Ig (EXOSC2 antibody) at dilution of 1:25.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules K-562, cellules LNCaP, cellules U2OS
• Résultats positifs en IHC: tissu hépatique humain, tissu de cancer de la peau humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below

Informations sur le produit

66099-1-Ig cible EXOSC2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EXOSC2 Protéine recombinante Ag7003
• Nom complet: exosome component 2
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC000747
• Symbole du gène: EXOSC2
• Identification du gène (NCBI): 23404
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snoRNA and snRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs [PMID:15346807]. EXOSC2 is a non-catalytic component of the RNA exosome complex that has 3'->5' exoribonuclease activity and involves in a multitude of cellular RNA processing and degradation events [PMID: 17545563].

Publications

Species	Application	Title
human		Cell; The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest.; Authors - Jakob Trendel
human	WB	Front Endocrinol (Lausanne); FGF2 Is Protective Towards Cisplatin-Induced KGN Cell Toxicity by Promoting FTO Expression and Autophagy.; Authors - Rongli Wang
human	WB	Reprod Biol Endocrinol; FTO protects human granulosa cells from chemotherapy-induced cytotoxicity.; Authors - Rongli Wang
human	WB	bioRxiv; Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication.; Authors - Tobias Moll
human	WB	J Oncol; Metformin Exhibits an Attractive Antineoplastic Effect on Human Endometrial Cancer by Regulating the Hippo Signaling Pathway; Authors - Meng Wang
human	WB	Life Sci Alliance; Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication; Authors - Tobias Moll

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Nikolaus (Verified Customer) (07-14-2022)

Exosc2 depletion is used as a control. Antibodies were incubated ON at 4C.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mESCs

EXOSC2 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:66099-1-Ig)

FH

Tsimafei (Verified Customer) (06-01-2022)

WT and Exosc2 depletion were compared. There is a clear reduction of Exosc2 signal upon the depletion. Though, the strength of the signla isn't great. Antibodies were incubated at 4C, ON.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mESCs

EXOSC2 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:66099-1-Ig)