Anticorps Monoclonal anti-ERAP2

ERAP2 Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

2B11A2

N° de cat : 67477-1-Ig

Synonymes

ERAP2, FLJ23633, FLJ23701, FLJ23807, L RAP, LRAP

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Galerie de données de validation

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  WB analysis of A549 using 67477-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 67477-1-Ig (ERAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67477-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67477-1-Ig (ERAP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67477-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67477-1-Ig (ERAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67477-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67477-1-Ig (ERAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67477-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67477-1-Ig (ERAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2, cellules HSC-T6

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67477-1-Ig cible ERAP2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ERAP2 Protéine recombinante Ag30061
• Nom complet: endoplasmic reticulum aminopeptidase 2
• Masse moléculaire calculée: 110 kDa
• Poids moléculaire observé: 110-120 kDa
• Numéro d’acquisition GenBank: BC065240
• Symbole du gène: ERAP2
• Identification du gène (NCBI): 64167
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ERAP2(Endoplasmic reticulum aminopeptidase 2) is also named as LRAP(Leukocyte-derived arginine aminopeptidase). It plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. ERAP2 can form heterodimers with ERAP1(PMID:15908954). Defects in the expression of ERAP2 may cause improper antigen processing, possibly leading to favor tumor escape from the immune surveillance. ERAP2 has the calculated molecular mass of 105-110 kDa, and apparent molecular mass of 120 kDa duo to the glycosylation.

Publications

Species	Application	Title
human	WB	Aging (Albany NY); ERAP2 as a potential biomarker for predicting gemcitabine response in patients with pancreatic cancer; Authors - Pian Yu