Anticorps Monoclonal anti-HIF2α/EPAS1

HIF2α/EPAS1 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

2F6B12

Avis (3)

N° de cat : 66731-1-Ig

Synonymes

bHLHe73, ECYT4, EPAS 1, EPAS1, HIF 1 alpha like factor, HIF 2 alpha, HIF2 alpha, HIF2A, HIF-2A, HIF2α, HIF-2α, HLF, Member of PAS protein 2, MOP2, PASD2

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Galerie de données de validation

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  WB analysis of HeLa using 66731-1-Ig

  untreated and cobalt chloride treated Hela cells were subjected to SDS PAGE followed by western blot with 66731-1-Ig (EPAS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66731-1-Ig

  untreated and cobalt chloride treated HepG2 cells were subjected to SDS PAGE followed by western blot with 66731-1-Ig (EPAS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 66731-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66731-1-Ig (EPAS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66731-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66731-1-Ig (EPAS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

66731-1-Ig cible HIF2α/EPAS1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: bovin, Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HIF2α/EPAS1 Protéine recombinante Ag24886
• Nom complet: endothelial PAS domain protein 1
• Masse moléculaire calculée: 96 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC051338
• Symbole du gène: EPAS1
• Identification du gène (NCBI): 2034
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Endothelial PAS domain-containing protein 1 (EPAS1), also known as Hypoxia-inducible factor 2-alpha (HIF2-alpha,HIF2A), is a transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. EPAS1 is expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.

Publications

Species	Application	Title
human	WB	Nat Commun; Extravillous trophoblast cell lineage development is associated with active remodeling of the chromatin landscape; Authors - Kaela M Varberg ; KD Validated
human	WB	Cell Mol Gastroenterol Hepatol; A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function.; Authors - Dayan Sun
bovine	WB,IF	Front Vet Sci; miRNA-150_R-1 mediates the HIF-1/ErbB signaling pathway to regulate the adhesion of endometrial epithelial cells in cows experiencing retained placenta; Authors - Chen Lv

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Balawant (Verified Customer) (12-25-2023)

I have used this antibody. this antibody is working great and have no bacground.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: renal cancer cell line (caki-1 and caki-2) and colon cancer cell (caco2)

FH

lUNFENG (Verified Customer) (01-27-2020)

IT IS NOT VERY GOOD.

• Applications: Immunofluorescence,

FH

Jie (Verified Customer) (01-27-2020)

Positive signal in heart samples, however, does not appear to be decreased in our KO mice model.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: mouse heart