Anticorps Polyclonal de lapin anti-HIF2α/EPAS1

HIF2α/EPAS1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

10

Avis (4)

N° de cat : 26422-1-AP

Synonymes

bHLHe73, ECYT4, EPAS 1, EPAS1, HIF 1 alpha like factor, HIF 2 alpha, HIF2 alpha, HIF2A, HIF-2A, HIF2α, HIF-2α, HLF, Member of PAS protein 2, MOP2, PASD2

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Galerie de données de validation

• 

  WB analysis using 26422-1-AP

  Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 26422-1-AP (EPAS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa traitées au chlorure de cobalt,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

26422-1-AP cible HIF2α/EPAS1 dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HIF2α/EPAS1 Protéine recombinante Ag24886
• Nom complet: endothelial PAS domain protein 1
• Masse moléculaire calculée: 96 kDa
• Poids moléculaire observé: 100-120 kDa
• Numéro d’acquisition GenBank: BC051338
• Symbole du gène: EPAS1
• Identification du gène (NCBI): 2034
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HIF2A, also named as EPAS1, is a 870 amino acid protein, which is expressed in most tissues, with highest levels in placenta, lung and heart. HIF2A colocalizes with HIF3A in the nucleus and speckles. HIF2A as a transcription factor involves in the induction of oxygen regulated genes. HIF2A binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. HIF2A regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. HIF2A may also play a role in the formation of the endothelium that gives rise to the blood brain barrier. The calculated molecular weight of HIF2A is 96 kDa, but In normoxia, HIF2A is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization. The modified HIf2A is about 100-120 kDa.

Publications

Species	Application	Title
	WB	Cell Mol Life Sci; Iron increases lipid deposition via oxidative stress-mediated mitochondrial dysfunction and the HIF1α-PPARγ pathway.; Authors - Chang-Chun Song
human	WB	Chemosphere; Organophosphate flame retardant TDCPP: A risk factor for renal cancer?; Authors - Xuan Zhou
human	WB,IF	Theranostics; Acidic Microenvironment Up-Regulates Exosomal miR-21 and miR-10b in Early-Stage Hepatocellular Carcinoma to Promote Cancer Cell Proliferation and Metastasis.; Authors - Xiao-Peng Tian
human	WB,IHC,CoIP	Cancer Biol Med; MIIP inhibits clear cell renal cell carcinoma proliferation and angiogenesis via negative modulation of the HIF-2α-CYR61 axis; Authors - Fengqi Yan
human	WB	Cell Death Dis; Regulatory mechanisms and function of hypoxia-induced long noncoding RNA NDRG1-OT1 in breast cancer cells; Authors - Hsing-Hua Chao
human	WB	Adv Sci (Weinh); STING Suppresses Mitochondrial VDAC2 to Govern RCC Growth Independent of Innate Immunity; Authors - Zhichuan Zhu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jingwen (Verified Customer) (01-27-2020)

It worked well with cancer tissue when I performed immunostaining.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Cancer tissue

FH

LUNFENG (Verified Customer) (01-27-2020)

NOT VERY GOOD

• Applications: Immunofluorescence,

FH

Jie (Verified Customer) (01-27-2020)

Positive staining in heart tissue, however, no difference were observed between KO mice and WT mice

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: mouse heart

FH

CHIHMIN (Verified Customer) (02-01-2019)

It works well in Western.

• Applications: Western Blot,