Anticorps Polyclonal de lapin anti-Endoglin/CD105

Endoglin/CD105 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 28117-1-AP

Synonymes

CD105, END, Endoglin, ENG, FLJ41744, HHT1, ORW, ORW1

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Galerie de données de validation

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  WB analysis using 28117-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28117-1-AP (Endoglin/CD105 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 28117-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28117-1-AP (Endoglin/CD105 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 28117-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28117-1-AP (Endoglin/CD105 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 28117-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28117-1-AP (Endoglin/CD105 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 28117-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28117-1-AP (Endoglin/CD105 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 28117-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 28117-1-AP (Endoglin/CD105 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 28117-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 28117-1-AP (Endoglin/CD105 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 28117-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28117-1-AP (Endoglin/CD105 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 28117-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28117-1-AP (Endoglin/CD105 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HUVEC, tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du sein humain, tissu de carcinome à cellules rénales humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 2 publications below

Informations sur le produit

28117-1-AP cible Endoglin/CD105 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Endoglin/CD105 Protéine recombinante Ag27822
• Nom complet: endoglin
• Masse moléculaire calculée: 71 kDa
• Poids moléculaire observé: 95 kDa
• Numéro d’acquisition GenBank: BC014271
• Symbole du gène: ENG
• Identification du gène (NCBI): 2022
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

Publications

Species	Application	Title
human	IHC	Mol Cancer Res; A TGFβ-dependent stromal subset underlies immune checkpoint inhibitor efficacy in DNA mismatch repair-deficient/microsatellite instability-high colorectal cancer.; Authors - Eisei Endo
human	IHC	Sci Rep; Public RNA-seq data-based identification and functional analyses reveal that MXRA5 retains proliferative and migratory abilities of dental pulp stem cells; Authors - Kazuma Yoshida