Anticorps Monoclonal anti-ELF1

ELF1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

2D4A11

N° de cat : 67138-1-Ig

Synonymes

E74 like factor 1, ELF1

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Galerie de données de validation

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  WB analysis using 67138-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67138-1-Ig (ELF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 67138-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 67138-1-Ig (ELF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 67138-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67138-1-Ig (ELF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules A431, cellules HL-60, cellules PC-3, cellules Ramos

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67138-1-Ig cible ELF1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ELF1 Protéine recombinante Ag14689
• Nom complet: E74-like factor 1 (ets domain transcription factor)
• Masse moléculaire calculée: 619 aa, 67 kDa
• Poids moléculaire observé: 93-97 kDa
• Numéro d’acquisition GenBank: BC030507
• Symbole du gène: ELF1
• Identification du gène (NCBI): 1997
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ELF1, also named as ETS-related transcription factor Elf-1, is originally cloned from a human T-cell cDNA library by hybridization with a probe encoding the DNA binding domain (ETS domain) of the human Ets-1 cDNA. Based on its preferential expression in embryonic lymphoid organs (thymus and spleen), a wide variety of epithelial cells and fetal liver as well as in adult haematopoietic tissues, including thymus, spleen and bone marrow, Elf-1 emerged as a potential key regulator of haematopoietic gene expression. Consistent with this notion, Elf-1 has been shown to be a direct upstream regulator of genes important for haematopoiesis such as Scl, Fli-1, Lyl-1, Runx1 and Lmo2 . Elf-1 has also been shown to be important for blood vessel development, a process that is closely linked to early haematopoiesis during embryonic development. Elf-1 has been reported to take part in the transcriptional control of major regulators of blood vessel development such as Tie1, Tie2, angiopoietin-2, the vascular endothelial growth factor receptor 1 (VEGFR1), the endothelial nitric-oxide synthase (eNOS) and endoglin . Functional activity of Ets proteins is modulated at multiple levels. It is known that ELF-1 appears in the cytoplasm as a 80 KDa protein that is O -glycosylated and phosphorylated in order to be translocated into the nucleus where it can be detected as a 98 KDa protein. After dephosphorylation, the protein is degraded through the proteasome pathway. The inactive form of Elf-1 is an 80-kDa protein that lacks DNA-binding activity and is confined to the cytoplasm of the cell. Phosphorylation and O-linked glycosylation increase the molecular weight of Elf-1 to 98 kDa, the active form; 98 kDa Elf-1 binds to the promoter of the gene that codes for CD3ζ inducing its transcription.