Anticorps Recombinant de lapin anti-EIF6

EIF6 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

2G23

N° de cat : 82693-1-RR

Synonymes

2, b, b(2)gcn, B(2)GCN homolog, B4 integrin interactor, CAB, eIF 6, EIF3A, EIF6, gcn, ITGB4BP, p27(BBP), p27BBP

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Galerie de données de validation

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  WB analysis using 82693-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82693-1-RR (EIF6 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A375, cellules A549, cellules COLO 320, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82693-1-RR cible EIF6 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: EIF6 Protéine recombinante Ag0324
• Nom complet: eukaryotic translation initiation factor 6
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC001119
• Symbole du gène: EIF6
• Identification du gène (NCBI): 3692
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

p27(BBP/eIF6) is an evolutionarily conserved protein that was originally identified as p27(BBP), it functions as an interactor of the cytoplasmic domain of integrin 4 and as the putative translation initiation factor eIF6. p27BBP is found in two pools: one nuclear pool enriched in the perinucleolar region, and one cytoplasmic pool. p27BBP binds to the fibronectin type III domains of integrin 4 subunit (ITGB4), an important functional component of hemidesmosomes, and help link ITGB4 to the intermediate filament cytoskeleton. In vitro and in vivo studies demonstrated that p27BBP is essential for cell viability and has a primary function in the biogenesis of the 60S ribosomal subunit. p27BBP protein is increased in rapidly cycling cells and decreased in villous cells committed to apoptotic cell death. In dysplastic colorectal adenomas and carcinomas, p27BBP displayed a large increase of its nucleolar component and was associated with the nuclear matrix. In particular, p27BBP increased progressively from adenomas to carcinomas and was related to the tumor stage.