Anticorps Monoclonal anti-EIF3M

EIF3M Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

2A12B2

N° de cat : 66025-1-Ig

Synonymes

B5, EIF3M, Fetal lung protein B5, GA17, hfl B5, HFLB5, PCID1, PNAS 125

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Galerie de données de validation

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  WB analysis using 66025-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66025-1-Ig (EIF3M antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of HeLa using 66025-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66025-1-Ig (EIF3M antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66025-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66025-1-Ig (EIF3M antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 66025-1-Ig

  MCF7 cells were subjected to SDS PAGE followed by western blot with 66025-1-Ig (EIF3M antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 66025-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66025-1-Ig (EIF3M antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66025-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66025-1-Ig (EIF3M antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 66025-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66025-1-Ig (EIF3M antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 66025-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66025-1-Ig (EIF3M antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 66025-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66025-1-Ig (EIF3M antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of MCF-7 using 66025-1-Ig

  Immunofluorescent analysis of MCF-7 cells, using EIF3M antibody 66025-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules MCF-7, cellules NIH/3T3, cellules PC-12, cellules RAW 264.7
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66025-1-Ig cible EIF3M dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EIF3M Protéine recombinante Ag17965
• Nom complet: eukaryotic translation initiation factor 3, subunit M
• Masse moléculaire calculée: 374 aa, 43 kDa
• Poids moléculaire observé: 35-43 kDa
• Numéro d’acquisition GenBank: BC019103
• Symbole du gène: EIF3M
• Identification du gène (NCBI): 10480
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EIF3M gene encodes eukaryotic translation initiation factor (eIF) subunit M, also called GA17 or PCID1. eIF-3 complex is required for several steps in the initiation of protein synthesis, and recent studies indicate that regulation of oncogene expression and neoplastic transformation are controlled by eIF subunits. The most uncharacterized non-core subunit EIF3M was confirmed to be highly expressed in human cancer cell lines and colon cancer patient tissues and mediate regulation of tumorigenesis-related genes in human colon cancer.