Anticorps Monoclonal anti-EHMT2

• Phare
• Validé par KD/KO

EHMT2 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

8

CloneNo.

1A12E3

Avis (1)

N° de cat : 66689-1-Ig

Synonymes

BAT8, C6orf30, DKFZp686H08213, EHMT2, FLJ35547, G9A, H3 K9 HMTase 3, HLA B associated transcript 8, KMT1C, Lysine N methyltransferase 1C, NG36, NG36/G9a, Protein G9a

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Galerie de données de validation

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  WB analysis of HeLa using 66689-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66689-1-Ig (EHMT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66689-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66689-1-Ig (EHMT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66689-1-Ig

  WB result of EHMT2 antibody (66689-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EHMT2 transfected HEK-293 cells.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below

Informations sur le produit

66689-1-Ig cible EHMT2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EHMT2 Protéine recombinante Ag27477
• Nom complet: euchromatic histone-lysine N-methyltransferase 2
• Masse moléculaire calculée: 1210 aa, 132 kDa
• Poids moléculaire observé: 150 kDa and 170 kDa
• Numéro d’acquisition GenBank: BC018718
• Symbole du gène: EHMT2
• Identification du gène (NCBI): 10919
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cell Death Differ; Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration; Authors - Huaizhen Liang
human	WB	Nat Commun; PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression.; Authors - Xianchun Lan
human	WB	Am J Cancer Res; HSF2 regulates aerobic glycolysis by suppression of FBP1 in hepatocellular carcinoma.; Authors - Li-Na Yang
human	WB	iScience; Linkage of methionine addiction, histone lysine hypermethylation, and malignancy.; Authors - Jun Yamamoto
human	WB	bioRxiv; Distinct functions of EHMT1 and EHMT2 in cancer chemotherapy and immunotherapy; Authors - Zhihua Kang
mouse	WB	Front Cell Dev Biol; Emerin interacts with histone methyltransferases to regulate repressive chromatin at the nuclear periphery; Authors - Nicholas Marano

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hongtao (Verified Customer) (12-17-2021)

This antibody works very well for human cancer cell whole cell lysate with 10 ug total protein input.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human kidney cancer cell

EHMT2 Antibody Western Blot validation (1:1000 dilution) in human kidney cancer cell (Cat no:66689-1-Ig)