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Anticorps Monoclonal anti-EEF1D

EEF1D Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

3B1B11

N° de cat : CL594-60085

Synonymes

Antigen NY CO 4, EEF1D, EF 1 delta, EF 1D, EF1D, Elongation factor 1 delta, FLJ20897, FP1047

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated EEF1D Monoclonal antibody (CL594-60085)

    IF Staining of HeLa using CL594-60085

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 EEF1D antibody (CL594-60085, Clone: 3B1B11 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated EEF1D Monoclonal antibody (CL594-60085)

    IF Staining of HeLa using CL594-60085

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-60085 (EEF1D antibody) at dilution of 1:100.

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite®594-conjugated EEF1D Monoclonal antibody (CL594-60085)

    FC experiment of MCF-7 using CL594-60085

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human EEF1D (CL594-60085, Clone:3B1B11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en IFcellules HeLa,
Résultats positifs en cytométriecellules MCF-7

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-60085 cible EEF1D dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène EEF1D Protéine recombinante Ag0983
Nom complet eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
Masse moléculaire calculée 31 kDa
Poids moléculaire observé 35-40 kDa
Numéro d’acquisition GenBankBC007847
Symbole du gène EEF1D
Identification du gène (NCBI) 1936
Conjugaison CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 )

Protocole

Product Specific Protocols
IF protocol for CL594 EEF1D antibody CL594-60085Download protocol
Standard Protocols
Click here to view our Standard Protocols