Anticorps Monoclonal anti-ECM1

ECM1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

2D1A3

N° de cat : 68472-1-Ig

Synonymes

ECM1, extracellular matrix protein 1, Secretory component p85

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Galerie de données de validation

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  WB analysis using 68472-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 68472-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 68472-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 68472-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 68472-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules hTERT-RPE1, cellules A431, cellules K-562, cellules THP-1, plasma humain, tissu placentaire humain

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68472-1-Ig cible ECM1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: extracellular matrix protein 1
• Masse moléculaire calculée: 540 aa, 61 kDa
• Poids moléculaire observé: 60-80 kDa
• Numéro d’acquisition GenBank: BC023505
• Symbole du gène: ECM1
• Identification du gène (NCBI): 1893
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ECM1 (extracellular matrix protein 1), also known as URBWD. It is located in secreted, extracellular space, and extracellular matrix, which is mainly expressed in esophagus and gall bladder. The gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. The calculated molecular weight of ECM1 is 60 kDa, and this antibody can recognize the 63 kDa isoform of target, moreover, this protein may have glycosylation modification.