Anticorps Polyclonal de lapin anti-Drosha

• Phare
• Validé par KD/KO

Drosha Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 55001-1-AP

Synonymes

DROSHA, ETOHI2, HSA242976, p241, RANSE3L, Ribonuclease 3, Ribonuclease III, ribonuclease type III, nuclear, RN3, RNase III, RNASE3L, RNASEN

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Galerie de données de validation

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  WB analysis of HEK-293 using 55001-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55001-1-AP (Drosha antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 55001-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55001-1-AP (Drosha antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 55001-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55001-1-AP (Drosha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 55001-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55001-1-AP (Drosha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 55001-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55001-1-AP (Drosha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 55001-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55001-1-AP (Drosha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 55001-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55001-1-AP (Drosha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55001-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55001-1-AP (Drosha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below

Informations sur le produit

55001-1-AP cible Drosha dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ribonuclease type III, nuclear
• Masse moléculaire calculée: 159 kDa
• Poids moléculaire observé: 90 kDa, 151 kDa
• Numéro d’acquisition GenBank: NM_013235
• Symbole du gène: RNASEN
• Identification du gène (NCBI): 29102
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DROSHA, also named as RN3, RNASE3L, P241 and RNASEN, is double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. It is a component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, RNASEN/DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. RNASEN is involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. And it is involved in the formation of GW bodies. The antibody is specific to DROSHA. DROSHA/RNASEN has some transcription forms with MW 160kd, 151kd, 138kd and 90kd.

Publications

Species	Application	Title
human	WB	Sci Rep; The Fragment HMGA2-sh-3p20 from HMGA2 mRNA 3'UTR Promotes the Growth of Hepatoma Cells by Upregulating HMGA2.; Authors - Yuan Wang
human	WB	Am J Physiol Cell Physiol; Hydrogen sulfide rescues high glucose-induced migration dysfunction in HUVECs by upregulating miR-126-3p.; Authors - Wen-Long Xue
human	WB	J Gen Virol; HBV induced the discharge of intrinsic antiviral miRNAs in HBV-replicating hepatocytes via extracellular vesicles to facilitate its replication.; Authors - Qiaofang Chu ; KD Validated
mouse	WB	Biochem Biophys Res Commun; METTL3 regulates skeletal muscle specific miRNAs at both transcriptional and post-transcriptional levels.; Authors - Li-Ting Diao
		Acta Pharmacol Sin; Post-transcriptional modulation of protein phosphatase PPP2CA and tumor suppressor PTEN by endogenous siRNA cleaved from hairpin within PTEN mRNA 3'UTR in human liver cells.; Authors - Yu-En Gao
human	WB	Cell Death Dis; FoxP3-miR-150-5p/3p suppresses ovarian tumorigenesis via an IGF1R/IRS1 pathway feedback loop.; Authors - Qinkai Zhang