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Anticorps Polyclonal de lapin anti-Desmin

Desmin Polyclonal Antibody for FC (Intra), IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

N° de cat : CL647-16520

Synonymes

CMD1I, CSM1, CSM2, Cytoskeleton Marker, DES, desmin, FLJ12025, FLJ39719, FLJ41013, FLJ41793

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using CoraLite® Plus 647-conjugated Desmin Polyclonal an (CL647-16520)

    IF Staining of mouse heart using CL647-16520

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 647 Desmin antibody (CL647-16520) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using CoraLite® Plus 647-conjugated Desmin Polyclonal an (CL647-16520)

    IF Staining of mouse heart using CL647-16520

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 647 Desmin antibody (CL647-16520) at dilution of 1:200.

  • Flow cytometry (FC) experiment of C2C12 cells using CoraLite® Plus 647-conjugated Desmin Polyclonal an (CL647-16520)

    FC experiment of C2C12 using CL647-16520

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Desmin (CL647-16520) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en IFtissu cardiaque de souris,
Résultats positifs en cytométriecellules C2C12

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-16520 cible Desmin dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Desmin Protéine recombinante Ag9742
Nom complet desmin
Masse moléculaire calculée 470 aa, 54 kDa
Poids moléculaire observé 52-53 kDa
Numéro d’acquisition GenBankBC032116
Symbole du gène DES
Identification du gène (NCBI) 1674
Conjugaison CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.

Protocole

Product Specific Protocols
IF protocol for CL Plus 647 Desmin antibody CL647-16520Download protocol
Standard Protocols
Click here to view our Standard Protocols