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Anticorps Polyclonal de lapin anti-Desmin

Desmin Polyclonal Antibody for FC (Intra), IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

N° de cat : CL594-16520

Synonymes

Cell and organelle markers, CMD1I, CSM1, CSM2, Cytoskeleton Marker, DES, desmin, FLJ12025, FLJ39719, FLJ41013, FLJ41793

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using CoraLite®594-conjugated Desmin Polyclonal antibody (CL594-16520)

    IF Staining of mouse heart using CL594-16520

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 Desmin antibody (CL594-16520) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using CoraLite®594-conjugated Desmin Polyclonal antibody (CL594-16520)

    IF Staining of mouse heart using CL594-16520

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 Desmin antibody (CL594-16520) at dilution of 1:200.

  • Flow cytometry (FC) experiment of C2C12 cells using CoraLite®594-conjugated Desmin Polyclonal antibody (CL594-16520)

    FC experiment of C2C12 using CL594-16520

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Desmin (CL594-16520) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en IFtissu cardiaque de souris,
Résultats positifs en cytométriecellules C2C12

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-16520 cible Desmin dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Desmin Protéine recombinante Ag9742
Nom complet desmin
Masse moléculaire calculée 470 aa, 54 kDa
Poids moléculaire observé 52-53 kDa
Numéro d’acquisition GenBankBC032116
Symbole du gène DES
Identification du gène (NCBI) 1674
Conjugaison CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.

Protocole

Product Specific Protocols
IF protocol for CL594 Desmin antibody CL594-16520Download protocol
Standard Protocols
Click here to view our Standard Protocols