Anticorps Monoclonal anti-Desmin

Desmin Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

CloneNo.

2B1A11

N° de cat : 67793-1-Ig

Synonymes

Cell and organelle markers, CMD1I, CSM1, CSM2, Cytoskeleton Marker, DES, desmin, FLJ12025, FLJ39719, FLJ41013, FLJ41793

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Galerie de données de validation

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  WB analysis using 67793-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67793-1-Ig (Desmin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of mouse skeletal muscle using 67793-1-Ig

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67793-1-Ig (Desmin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 67793-1-Ig

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 67793-1-Ig (Desmin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 67793-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67793-1-Ig (Desmin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 67793-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67793-1-Ig (Desmin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human colon using 67793-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Desmin antibody (67793-1-Ig, Clone: 2B1A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue).

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  IF Staining of human appendicitis using 67793-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Desmin antibody (67793-1-Ig, Clone: 2B1A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse skeletal muscle using 67793-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Desmin antibody (67793-1-Ig, Clone: 2B1A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de porc, côlon de porc, muscle squelettique de porc, tissu de muscle squelettique de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de côlon humain, tissu de muscle squelettique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de côlon humain, tissu d'appendicite humain, tissu de muscle squelettique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 5 publications below

Informations sur le produit

67793-1-Ig cible Desmin dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Desmin Protéine recombinante Ag9675
• Nom complet: desmin
• Masse moléculaire calculée: 470 aa, 54 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC032116
• Symbole du gène: DES
• Identification du gène (NCBI): 1674
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.

Publications

Species	Application	Title
mouse	WB	Gastroenterology; Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells; Authors - Decai Wang
rat	IF	Mater Today Bio; Engineered muscle from micro-channeled PEG scaffold with magnetic Fe3O4 fixation towards accelerating esophageal muscle repair; Authors - Yang Luo
rat	IF	Exp Cell Res; Bone marrow-derived mesenchymal stem cells transplantation attenuates renal fibrosis following acute kidney injury by repairing the peritubular capillaries; Authors - Weifeng Hu
mouse	IF	DNA Cell Biol; Single-Cell RNA Sequencing Technology Revealed the Pivotal Role of Fibroblast Heterogeneity in Angiotensin II-Induced Abdominal Aortic Aneurysms.; Authors - Yingzheng Weng
rat	WB,IF	Mol Cell Biochem; Testosterone attenuates senile cavernous fibrosis by regulating TGFβR1 and galectin-1 signaling pathways through miR-22-3p; Authors - Zongren Hu
rat	WB	J Sex Med; Caveolin-1 scaffolding domain peptide prevents corpus cavernosum fibrosis and erectile dysfunction in bilateral cavernous nerve injury-induced rats; Authors - Yuhang Xi