Anticorps Polyclonal de lapin anti-DPP4/CD26

DPP4/CD26 Polyclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, FC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 29403-1-AP

Synonymes

ADABP, ADCP 2, ADCP2, CD26, dipeptidyl peptidase 4, Dipeptidyl peptidase IV, DPP IV, DPP4, DPPIV, T cell activation antigen CD26, TP103

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Galerie de données de validation

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  WB analysis using 29403-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29403-1-AP (DPP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of HT-29 using 29403-1-AP

  1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human DPP4/CD26 (29403-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules HT-29
• Résultats positifs en cytométrie: cellules HT-29,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

29403-1-AP cible DPP4/CD26 dans les applications de WB, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DPP4/CD26 Protéine recombinante Ag30160
• Nom complet: dipeptidyl-peptidase 4
• Masse moléculaire calculée: 88 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC013329
• Symbole du gène: DPP4
• Identification du gène (NCBI): 1803
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580). In addition to the 110 kDa monomeric form, a 290 kDa active dimeric form and a 60 kDa fragment of CD26 have also been reported (PMID: 9654125; 7905271).

Publications

Species	Application	Title
mouse	WB	Front Allergy; High-fat diet and palmitic acid amplify airway type 2 inflammation; Authors - Kris Genelyn Dimasuay
mouse	WB	Biomed Pharmacother; Fructus Lycii and Salvia miltiorrhiza Bunge extract attenuate oxidative stress-induced photoreceptor ferroptosis in retinitis pigmentosa; Authors - Yijing Yang
human	WB	Anal Chem; Ultrasensitive Fluorescent Probes Reveal an Adverse Action of Dipeptide Peptidase IV and Fibroblast Activation Protein during Proliferation of Cancer Cells; Authors - Qiuyu Gong