Anticorps Recombinant de lapin anti-DMT1

DMT1 Recombinant Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

1I13

Avis (1)

N° de cat : 81609-1-RR

Synonymes

DCT1, Divalent cation transporter 1, Divalent metal transporter 1, DMT 1, DMT1, FLJ37416, NRAMP 2, NRAMP2, SLC11A2

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Galerie de données de validation

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  WB analysis using 81609-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81609-1-RR (DMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 81609-1-RR

  IP result of anti-DMT1(IP:81609-1-RR, 4ug; Detection:81609-1-RR 1:2000) with HEK-293 cells lysate 1480 ug.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Caco-2, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules HuH-7, cellules Neuro-2a
• Résultats positifs en IP: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

81609-1-RR cible DMT1 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DMT1 Protéine recombinante Ag14314
• Nom complet: solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2
• Masse moléculaire calculée: 568 aa, 62 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC002592
• Symbole du gène: SLC11A2
• Identification du gène (NCBI): 4891
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC11A2 (also known as DMT1, Nramp2, and DCT1) is a member of the divalent cation transporters that plays a central role in iron homeostasis. SLC11A2 is widely expressed in many tissues including brain, kidney, testis, duodenum and placenta. As a membrane protein, SLC11A2 is localized on the apical membrane of enterocytes as well as in transferrin-cycle endosomes. Four isoforms of SLC11A2 exist due to the alternative splicing. They differ at the NH2 and COOH termini but share a common central domain. A variety of molecular weights of SLC11A2 in western blot analysis, ranging from 50 to 100 kDa, has been reported in different cells and species. The differences in molecular weights may be attributed to the level of glycosylation, proteolysis, or the membrane protein itself. This antibody detected various forms of SLC11A2 of 45-100 kDa.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Amandine (Verified Customer) (02-06-2024)

With 20ug of protein denatured or not, 1H30 RT 1/10000, secondary antibody 2h 1/5000. Chemiluminescence revelation, 6min of exposition

• Applications: Western Blot
• Primary Antibody Dilution: 1/10000
• Cell Tissue Type: ImCC

DMT1 Antibody Western Blot validation (1/10000 dilution) in ImCC (Cat no:81609-1-RR)